Air-exposed Tissue Culture of Human Middle Ear Epithelium and Meatal Epidermis: a Method to Study the Advancing Front of Cholesteatoma

Authors: Albers-Op 'T Hof B.M.1; Peek F.A.W.1; Huisman M.A.1; Grote J.J.1

Source: Acta Oto-Laryngologica, Volume 122, Number 7, 1 October 2002 , pp. 720-725(6)

Publisher: Informa Healthcare

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content

Abstract:

The suitability of an air-exposed culture model consisting of a collagen matrix was investigated for constructing an advancing front (AF) of human middle ear epithelium (MEE) and meatal epidermis (ME). Three different culture settings were used: (i) MEE; (ii) ME; and (iii) AF (MEE+ME). Small tissue biopsies were placed on a fibroblast-populated collagen matrix and grown at the air-liquid interface. After 3 weeks of culture, the MEE and ME outgrowth was differentiated. Light, scanning electron and transmission electron microscopy showed no visible differences compared to native MEE and ME. Cytokeratin 8 and cytokeratin 10 expressions were comparable to the expression seen in the native MEE and ME tissues. Proliferation, which was demonstrated by the expression of Ki-67, was present in the basal layers of cultured MEE and ME. A double layer of cells in which the ME covered the MEE formed the AF. In the AF, the MEE and ME showed the same morphological and immunohistochemical characteristics as in their native tissues. The results of the study show that this in vitro system is a well-defined model system offering the possibility to study the effects of external stimuli on the different epithelia of the AF involved in the pathogenesis of cholesteatoma.

Keywords: AIR-LIQUID; INTERFACE; CHRONIC MIDDLE EAR DISEASE; COLLAGEN GEL; CYTOKERATIN; IMMUNOHISTOCHEMISTRY; KERATINOCYTE .

Document Type: Research article

Affiliations: 1: Ear, Nose & Throat Department, Leiden University Medical Center, Leiden, The Netherlands

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$35.59 plus tax      Refund Policy

 

OR

Back to top

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages.
Page Help Click here for Page Help
Shopping cart
Tools
Sign in






Need to register?
Sign up here
Text size: A | A | A | A