Measurement of Albumin in Nasal Lavage Using the Technique of Chemiluminescent Immunoassay

Authors: Browning, S. T.; Housley, D. G.; Weeks, I.

Source: Acta Oto-Laryngologica, Volume 119, Number 4, 23 June 1999 , pp. 492-496(5)

Publisher: Informa Healthcare

Buy & download fulltext article:

OR

Price: $55.00 plus tax (Refund Policy)

Abstract:

A method has been developed that uses chemiluminescent acridinium esters rather than radioactive iodine in an immunoassay for albumin. Albumin is a protein derived from plasma sources found in nasal fluids. As such, it can be used as an important marker of plasma exudation in experimental rhinology. The assay was developed as an alternative to radioimmunoassay for a number of reasons: chemiluminescent assays do not require radioactive materials and the complex safety related aspects of their usage; the assays are easy to perform and do not require expensive equipment; and the assay is capable of the sensitivity required to detect very small changes in albumin concentrations in nasal fluids. The assay measures albumin with a sensitivity of 1.2 ng/ml. The working range of the assay is 1.0-23.7 g/ml with an interassay coefficient of variation ≤15%. This working range encompasses the range of albumin usually found in nasal lavage samples from normal volunteers.

Document Type: Regular Paper

DOI: http://dx.doi.org/10.1080/00016489950181053

Publication date: June 23, 1999

More about this publication?
Related content

Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content

Text size:

A | A | A | A
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages. print icon Print this page