Authors: Malling, Tine Halsen¹; Sigsgaard, Torben²; Andersen, Helle Raun³; Frischknecht, Lone⁴; Deguchi, Yoji⁵; Skadhauge, Lars⁶; Sherson, David⁷; Thomsen, Gert⁸; Bælum, Jesper⁹; Pedersen, Jacob Krabbe²; Omland, Øyvind¹⁰

Source: Scandinavian Journal of Clinical and Laboratory Investigation, Volume 69, Number 2, April 2009 , pp. 295-302(8)

Publisher: Informa Healthcare

Abstract:

Objective. Glutathione peroxidase 1 (GPX1) is one of the major oxidative enzymes. Our aim was to characterize factors influencing its activity and to determine whether or not the activity is associated with asthma. Material and methods. Serum selenium concentration was measured, GPX1 polymorphisms were genotyped and smoking history was obtained in a Danish population-derived case-base cohort of 1,191 subjects designed to evaluate risk factors for asthma. GPX1 activity was measured in 134 male and 164 female subjects equally distributed according to genotype of GPX1. Among these subjects, 82 (28 %) had doctor-diagnosed asthma. Results. The average serum selenium concentration was too low for optimal enzyme activity (mean (SE), 83.4 (0.76) ng/mL). GPX1 activity in men was lower than in women, 52.6 (0.66) and 56.4 (0.59) U/g protein, respectively (p<0.001). In men, activity was positively associated with serum selenium concentration (p = 0.005) and negatively associated with both active smoking (p = 0.009) and exposure to environmental tobacco smoke (p = 0.02). In women, activity was associated with genotypes with 59.2 (1.4), 56.0 (1.4) and 54.2 (1.4) U/g protein in the homozygote wild-type, the heterozygote and the homozygote variant type, respectively (p = 0.001). Doctor-diagnosed asthma was unrelated to GPX1 activity in either sex. Conclusion. Determinants for activity in the oxidative enzyme GPX1 show marked differences between the sexes, but the activity is not associated with asthma. Sex ought to be taken into consideration when analysing the activity of the enzyme.

Keywords: Asthma; epidemiology; glutathione peroxidase activity; glutathione peroxidase polymorphism; selenium; smoking

Document Type: Research article

DOI: http://dx.doi.org/10.1080/00365510802632155

Affiliations: 1: Department of Occupational Medicine, Aalborg Hospital, Denmark 2: Institute of Public Health, University of Århus, Denmark 3: Institute of Public Health, University of Southern Denmark, Odense, Denmark 4: Department of Clinical Biochemistry, Vejle Hospital, Denmark 5: School of Nursing, Faculty of Medical Sciences, University of Fukui, Japan 6: Department of Occupational and Environmental Medicine, Hospital of Southern Jutland, Denmark 7: Department of Occupational Medicine, Vejle Hospital, Denmark 8: Department of Occupational Medicine, Ribe County Hospital, Denmark 9: Department of Occupational Medicine, Odense University Hospital, Denmark 10: Department of Occupational Medicine, Aalborg Hospital, Denmark,Institute of Public Health, University of Århus, Denmark

Publication date: 2009-04-01

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