Validation of inductively coupled plasma atomic emission spectrometry technique (ICP-AES) for multi-element analysis of trace elements in human serum

Authors: Rahil-Khazen R.; Henriksen H.; Bolann B.J.; Ulvik R.J.

Source: Scandinavian Journal of Clinical and Laboratory Investigation, Volume 60, Number 8, 27 December 2000 , pp. 677-686(10)

Publisher: Informa Healthcare

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Abstract:

The use of inductively coupled plasma atomic emission spectrometry (ICP-AES) for the simultaneous determination of Al, B, Ba, Be, Cd, Co, Cr, Cu, Fe, Li, Mn, Ni, Pb, Se, Sr and Zn in human serum in a clinical laboratory was validated. Samples were digested and then analysed using yttrium as an internal standard and a serum-matched calibration standard. The criteria used to assess the analytical performance of the ICP-AES were detection and quantification limits, linearity, sensitivity, recovery, interference from alkali and acid, trueness and precision. Detection limits were 0.002–0.003

mol/L for Mn, Sr, Ba, and Cd; 0.014–0.07

mol/L for Co, Zn, Fe, Be, Li, Pb, Cu, Ni, and Cr; and 0.2–0.9

mol/L for B, Se, and Al. Trueness, as controlled by analysis of bovine serum certified reference material, was acceptable for Co, Cu, Se and Zn, while Fe was 5.1% and Mn 6.2% below the lowest limit of the certified material interval. We conclude that ICP-AES can be used for multi-element analysis of B, Ba, Cu, Fe, Li, Se, Sr and Zn in serum. Serum levels of Al, Be and Co were below the detection limits while serum levels of Cd, Cr, Ni and Pb were below the quantification limits of the ICP-AES. These trace metals cannot be analysed as routine by the ICP-AES. However, in cases of intoxication with elevated serum concentrations mean recovery of 100±10% was obtained at an addition of 2.22

mol/L for Al, 0.11

mol/L for Be, 0.03

mol/L for Co, 0.39

mol/L for Cr, 0.14

mol/L for Ni, and 0.12

mol/L for Pb.

Keywords: INDUCTIVELY COUPLED PLASMA ATOMIC EMISSION SPECTRO; METHOD VALIDATION; TRACE ELEMENT ANALYSIS; TRACE ELEMENT SERUM LEVELS

Language: English

Document Type: Research article

Affiliations: 1: Institute of Clinical Biochemistry, Haukeland University Hospital, Bergen, Norway

Publication date: 2000-12-27

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