IngentaConnect Leptin and leptin binding activity in the preovulatory follicle o...

Authors: Fedorcsák P.¹; Storeng R.²; Dale P.O.²; Tanbo T.²; Torjesen P.³; Urbancsek J.⁴; Åbyholm T.²

Source: Scandinavian Journal of Clinical and Laboratory Investigation, Volume 60, Number 8, 27 December 2000 , pp. 649-655(7)

Publisher: Informa Healthcare

Abstract:

To investigate the clinical importance of leptin's intraovarian effects, we studied the concentration of leptin and leptin binding activity in the plasma and in the follicular fluid of PCOS patients (n=20; median BMI: 27.1 kg/m², range 19.7–36.3) undergoing controlled ovarian stimulation with long-term GnRH agonist, recombinant FSH, and in vitro fertilization. Follicular fluid and blood samples were collected during follicle aspiration for IVF. Total leptin concentration was measured by radioimmunoassay, and specific leptin binding activity was accessed by a gel filtration column assay. Follicular fluid and plasma leptin levels were similar (median 1135 pmol/l vs. 1409 pmol/l; p=0.81). Follicular fluid to plasma leptin ratio was independently associated with cumulative FSH dose (r=0.63; p=0.006) and insulin resistance index (r=–0.45; p=0.04). Specific leptin binding activity was higher in the plasma than in the follicular fluid [median 7.94% vs. 3.49%; p<0.001]. When multivariate analysis was used to predict FSH consumption, only follicular fluid leptin levels were significantly associated with cumulative FSH dose (r=0.46; p=0.04). We infer that at least in part by increased intrafollicular leptin levels, obesity directly affects ovarian function in PCOS, and may induce a relative resistance to gonadotropin stimulation. This intraovarian effect of leptin can be even more profound because of low leptin binding activity in the preovulatory follicle of obese patients.

Keywords: FOLLICULAR FLUID; INSULIN RESISTANCE; LEPTIN; LEPTIN BINDING ACTIVITY; POLYCYSTIC OVARY SYNDROME

Language: English

Document Type: Research article

Affiliations: 1: Department of Obstetrics and Gynecology, National Hospital, University of Oslo, Oslo, Norway and First Department of Obstetrics and Gynecology, Semmelweis University Medical School, Budapest, Hungary 2: Department of Obstetrics and Gynecology, National Hospital, University of Oslo, Oslo, Norway 3: Hormone Laboratory, Aker University Hospital, Oslo, Norway 4: First Department of Obstetrics and Gynecology, Semmelweis University Medical School, Budapest, Hungary;

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Leptin and leptin binding activity in the preovulatory follicle of polycystic overy syndrome patients