Authors: Franke, Axel¹; Lante, Wolfgang²; Kurig, Edmond³; Zoeller, Lothar³; Kollig, Erwin⁴; Markewitz, Andreas²

Source: Scandinavian Cardiovascular Journal, Volume 43, Number 2, April 2009 , pp. 136-144(9)

Publisher: Informa Healthcare

Abstract:

Objectives. Interferon gamma (IFN-gamma) synthesis in peripheral blood mononuclear cells (PBMCs) is suppressed after major surgical trauma. Interleukin-12 (IL-12) has been shown to stimulate IFN-gamma-synthesis. We hypothesised that exogenous IL-12 can increase perioperative pro-inflammatory cytokine release. We therefore assessed the effect of IL-12 on IFN-gamma-synthesis and pro-inflammatory cytokine release in vitro before and after cardiac surgery. Design. In this prospective study, PBMCs from 20 elective cardiac surgery patients were stimulated for 24 hours with staphylococcal enterotoxin B and lipopolysaccharide before surgery (d0) and on the 1st (d1), 3rd (d3) and 5th (d5) postoperative days. IL-12 was added at each time point investigated. IFN-gamma, IL-6, tumour necrosis factor-alpha (TNF-alpha), IL-2, IL-4, IL-5, and IL-10 concentrations were assayed. Results. IFN-gamma-synthesis was significantly reduced at d1, d3 and d5. When IL-12 was added, IFN-gamma-synthesis returned to preoperative levels at d1, d3 and d5. Neither IL-6 nor TNF-alpha-synthesis was influenced by IL-12. Conclusions. IFN-gamma synthesis is significantly reduced after major surgical trauma. IL-12 increases IFN-gamma-synthesis before and after surgery without influencing pro-inflammatory cytokine synthesis.

Keywords: Interleukin-12; major surgical trauma; immune function; T-cell suppression; IFN-gamma-synthesis; cardiac surgery

Document Type: Research article

DOI: http://dx.doi.org/10.1080/14017430802409699

Affiliations: 1: Department of Cardiovascular Surgery, German Armed Forces Central Hospital, Koblenz, Germany,Department of Trauma Surgery, German Armed Forces Central Hospital, Koblenz, Germany 2: Department of Cardiovascular Surgery, German Armed Forces Central Hospital, Koblenz, Germany 3: Department of Immunology, Central Institute of the German Armed Forces Medical Service, Koblenz, Germany 4: Department of Trauma Surgery, German Armed Forces Central Hospital, Koblenz, Germany

Publication date: 2009-04-01

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