Authors: Martin Westerberg¹; Anders Bengtsson²; Anne Ricksten³; Anders Jeppsson¹

Source: Scandinavian Cardiovascular Journal, Volume 38, Number 5, October 2004 , pp. 312-317(6)

Publisher: Informa Healthcare

Abstract:

Background--Tumor necrosis factor- (TNF-), a key factor in the inflammatory cascade, has been implicated in coronary artery disease. Two biallelic polymorphisms in the TNF gene locus (TNFA at position -308 and TNFB at +252) may influence TNF- production. Individuals with the rare TNFA2 allele or TNFB2 homozygosity have augmented TNF- production. We investigated the genotypes associated with increased TNF- production in coronary artery bypass grafting (CABG) patients and if these genotypes influence the magnitude of the postoperative inflammatory response.

Methods--TNF gene polymorphisms were analyzed by multiplex fluorescent solid-phase minisequencing in 86 CABG patients. Plasma concentrations of TNF-, IL-6 and C3a and C-reactive protein (CRP) were analyzed before and after surgery in 45 of the patients and compared with genetically high and low TNF- producers.

Results--Thirty percent of the patients carried the TNFA2 allele and 45% were TNFB2 homozygous. The allelic frequencies were TNFA1/TNFA2 = 0.84/0.16 and TNFB1/TNFB2 = 0.32/0.68. Pre- and postoperative levels of TNF-, IL-6, C3a and CRP did not differ significantly between genetically high and low TNF- producers.

Conclusions--The frequency of high TNF- producing genotypes in a CABG population was comparable to that previously reported from normal populations. Furthermore, we found no evidence that the investigated TNF- gene polymorphisms influence postoperative inflammatory response after uncomplicated coronary surgery.

Keywords: CABG; gene polymorphism; systemic inflammatory response; tumor necrosis factor

Document Type: Research article

DOI: http://dx.doi.org/10.1080/14017430410031795

Affiliations: 1: Department of Cardiothoracic Surgery Sahlgrenska University Hospital Gothenburg Sweden 2: Department of Anesthesia and Intensive Care Sahlgrenska University Hospital Gothenburg Sweden 3: Department of Clinical Chemistry and Laboratory Medicine Sahlgrenska University Hospital Gothenburg Sweden

Publication date: 2004-10-01

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