Penetration of Gatifloxacin Eye Drops into the Aqueous Humor in Humans
Source: Ocular Immunology and Inflammation, Volume 15, Number 4, July 2007 , pp. 309-313(5)
Publisher: Informa Healthcare
Abstract:The authors topically administered gatifloxacin (GFLX) into the eye before cataract surgery and measured the concentrations of this agent to determine its penetration into aqueous humor. Seventy-seven patients with age-related cataracts who underwent cataract surgery were enrolled in this study. They received 0.3% GFLX ophthalmic solution 4 times at 30-min intervals, beginning 2 h before surgery. Aqueous humor was aspirated from the anterior chamber and assayed for GFLX concentration using high-performance liquid chromatography. The mean intraoperative GFLX concentration in aqueous humor was 0.485 ± 0.328 g/mL. GFLX level was 0.573 ± 0.367 g/mL in elderly patients, at least 70 years of age, and was significantly higher than that (0.322 ± 0.135 g/mL) in the patients less than 70 years old. This concentration was close to or higher than the minimum inhibitory concentrations required to inhibit the growth of 90% of major pathogens of endophthalmitis (MIC90), such as Staphylococcus aureus, Streptococcus pneumoniae, and Enterococcus faecalis associated with poor prognosis, other than Staphylococcus epidermidis, Pseudomonas aeruginosa, and MRSA (methicillin-resistant S. aureus) in vitro. The GFLX concentrations found in aqueous humor samples were sufficient to kill bacteria other than S. epidermidis, P. aeruginosa, and MRSA in vitro.
Document Type: Research Article
Affiliations: 1: Yokosuka Chuoh Eye Clinic, Kanagawa, Japan,Department of Ophthalmology and Visual Science, Yokohama City University School of Medicine, Kanagawa, Japan 2: Yokohama Sky Eye Clinic, Kanagawa, Japan 3: Department of Ophthalmology and Visual Science, Yokohama City University School of Medicine, Kanagawa, Japan 4: Shin Nippon Biomedical Laboratories, Ltd., Wakayama, Japan
Publication date: 2007-07-01