Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells

Authors: Koch, Thomas¹; Thomsen, Preben²; Betts, Dean³

Source: Cytotherapy, Volume 11, Number 4, July 2009 , pp. 443-447(5)

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Abstract:

Background aims A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. Methods Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. Results and conclusions Our results indicate that PrepaCyte®-EQ medium is superior to Ficoll-Paque™ PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.

Keywords: Cord blood; horse; isolation; mesenchymal stromal cells

Document Type: Research article

DOI: http://dx.doi.org/10.1080/14653240902887259

Affiliations: 1: Department of Biomedical Sciences, University of Guelph, Ontario, Guelph, Canada,Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Denmark 2: Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Denmark 3: Department of Biomedical Sciences, University of Guelph, Ontario, Guelph, Canada

Publication date: 2009-07-01

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