Early Detection of Response to Hydroxyurea Therapy in Patients with Sickle Cell Anemia
Source: Hemoglobin, Volume 34, Number 5, October 2010 , pp. 424-429(6)
Publisher: Informa Healthcare
Abstract:Red blood cells (RBC) and reticulocyte parameters were determined on peripheral blood from a subset of patients enrolled in the multicenter study of hydroxyuea (HU) in sickle cell anemia. Multiple blood samples were obtained every 2 weeks. Cellular indices were measured by flow cytometry. Generalized linear models were used to determine the relationship between the longitudinal trajectories of RBC and reticulocyte indices and HU usage. There was a significant relationship between HU usage and most of the RBC and reticulocyte indices. Hydroxyurea produced higher value trajectories than those generated by placebo usage for the hemoglobin (Hb) content of both the RBCs and reticulocytes and for the mean corpuscular volume (MCV) of reticulocytes. These changes were first detected 10 weeks after starting HU and before the increase in Hb F levels. The data suggest that subtle and early markers of response to HU reside in the hemogram.
Document Type: Research Article
Affiliations: 1: 1Cardeza Foundation for Hematologic Research, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA 2: 2Maryland Medical Research Institute, Baltimore, Maryland, USA 3: 3The Children's Hospital, Boston, Maryland, USA 4: 4Siemens Healthcare Diagnostics Inc., TarrytownNew York, USA 5: 5National Heart, Lung and Blood Institute, Bethesda, Maryland, USA
Publication date: October 1, 2010