Authors: Wang, Xu¹; Sun, Zhengyi¹; Wang, Jing¹; Nan, Guoxin²; Ma, Yanchao¹; Wang, Shuanke¹; Xia, Yayi¹; Zhang, Youcheng¹

Source: International Journal of Neuroscience, Volume 119, Number 1, August 2009 , pp. 141-154(14)

Publisher: Informa Healthcare

Abstract:

We investigated whether X-ray radiation induced apoptosis of the proliferative ependymal cells (ECs) in adult rats with spinal cord injury (SCI) and the effect of X-ray radiation on the proliferative activities of ECs. A rat model with SCI was developed and used to determine the proliferation and apoptosis of ECs in the spinal cords after X-ray exposure. TUNEL assay and BrdU incorporation were used to detect apoptosis and proliferation respectively. We found that there were few TUNEL-positive cells in proliferative ependymal zone (EZ) after SCI except at the epicenter, and approximately half of the irradiated ECs became TUNEL-positive. However, these radiated ECs did not lose their proliferative activity until 1 week later and started to decrease rapidly after 1 week. The observation suggested that only part of ECs were sensitive to radiation and the nonsensitive cells continued their mitosis process. These findings indicated that X-ray exposure of the rats with SCI in early stage induced apoptosis of the proliferative ECs and partially inhibited their proliferative activities.

Keywords: apoptosis; ependymal cells; proliferation; spinal cord injury

Document Type: Research article

DOI: http://dx.doi.org/10.1080/00207450802540599

Affiliations: 1: 1Department of Orthopaedics, the Second Hospital of Lanzhou University, Lanzhou, Gansu, 730030, China 2: 2Battle Surgery Research Institute, The Third Military Medical University, Chongqing, 400042, China

Publication date: 2009-08-01

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