Quantitative Immunohistochemical Analysis of Nitric Oxide Synthases and Apoptosis Regulator Proteins in the Fetal Rat Brain Following Maternal Uterine Artery Ligation

Authors: Uysal, Aysegul; Oktem, Gulperi; Yilmaz, Ozlem; Uslu, Serap; Aktug, Huseyin; Yurtseven, Mine Ertem

Source: International Journal of Neuroscience, Volume 118, Number 6, June 2008 , pp. 891-901(11)

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Abstract:

The purpose of this study was to determine the relation between nitric oxide synthases (calcium-independent iNOS and calcium-dependent eNOS) and apoptosis regulator proteins (anti-apoptotic Bcl-2, pro-apoptotic p53) of fetal rat brain in experimental intrauterine growth retardation (IUGR) model via quantitative immunohistochemistry. Cortical zone of parietal cerebral cortex and ventricular zone of third ventricle were studied following bilateral uterine artery ligation on gestational day 18. Significant increase in iNOS immunoreactivity was determined in parietal cerebral cortex and ventricular zones as eNOS immunoreactivity increased in ventricular zone of IUGR group. Bcl-2 expression was significantly decreased in ventricular zone; whereas cortical zone of IUGR group expressed p53 immunoreactivity.

Keywords: apoptosis; fetal brain; intrauterine growth retardation (IUGR); nitric oxide synthases (NOS); uterine artery ligation

Document Type: Research article

DOI: http://dx.doi.org/10.1080/00207450701769364

Affiliations: 1: Department of Histology and Embryology, Ege University, Faculty of Medicine, Izmir, Turkey

Publication date: 2008-06-01

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