Oxidative stress implication in a new ex-vivo cardiac concordant xenotransplantation model
Authors: Charniot, Jean-Christophe1; Bonnefont-Rousselot, Dominique2; Albertini, Jean-Paul1; Zerhouni, Khaled1; Dever, Sylvie2; Richard, Isabelle3; Nataf, Patrick4; Pavie, Alain4; Monsuez, Jean-Jacques1; Delattre, Jacques2; Artigou, Jean-Yves1
Source: Free Radical Research, Volume 41, Number 8, November 2007 , pp. 911-918(8)
Publisher: Informa Healthcare
Abstract:Xenotransplantation (XT) reveals a growing interest for the treatment of cardiomyopathy. The major barrier is an acute vascular rejection due to an acute humoral rejection. This pathogenesis is a difficult issue and in order to elaborate means for its prevention, we analysed the implication of oxidative stress (OS) on hearts from mini-pigs followed by reperfusion with either autologous or human blood in an attempt to simulate xenotransplantation.
About 14 hearts were studied after a Langendorff blood reperfusion: allografts with autologous blood (n == 7) or xenografts with human blood (n == 7). Blood samples were drawn from the coronary sinus to assess ischemia and OS.
In xenografts, arrhythmias occurred more frequently (p < 0.01, left ventricular systolic pressure decreased more significantly (p < 0.05), thiobarbituric acid-reactive substances concentrations increased at 30 min (0.7 ± 0.1 vs. 2.4 ± 0.3 mmol/l; p < 0.05) while vitamin A levels decreased (p < 0.05).
XT was associated with a significant increase in ischemic injury and OS production. OS might play an eminent role in hyperacute humoral rejection.
Document Type: Research Article
Affiliations: 1: 1AP-HP, Hospital Avicenne, Department of Cardiology, Université de Paris XIII, Faculté de medicine de Bobigny, Paris XIII, France 2: 2Federation of Biochemistry, GH Pitié-Salpêtrière (AP-HP), Paris, France 3: 3Department of Biochemistry (EA 3617), Faculty of Pharmacy, Paris 5, France 4: 4Department of Cardiac Surgery, GH Pitié-Salpêtrière (AP-HP) Hospital, Paris, France
Publication date: November 1, 2007
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- In this Subject: Biology , Chemistry (General)
- By this author: Charniot, Jean-Christophe ; Bonnefont-Rousselot, Dominique ; Albertini, Jean-Paul ; Zerhouni, Khaled ; Dever, Sylvie ; Richard, Isabelle ; Nataf, Patrick ; Pavie, Alain ; Monsuez, Jean-Jacques ; Delattre, Jacques ; Artigou, Jean-Yves