Aromatic Trap Analysis of Free Radicals Production in Experimental Collagen-induced Arthritis in the Rat: Protective Effect of Glycosaminoglycans Treatment
Source: Free Radical Research, Volume 37, Number 3, 1 January 2003 , pp. 257-268(12)
Publisher: Informa Healthcare
Abstract:Many findings demonstrated that Glycosaminoglycans (GAGs) and Proteoglycans (PGs) possess antioxidant activity. Collagen-induced arthritis (CIA) is an experimental animal model similar to human rheumatoid arthritis (RA) in which free radicals are involved. Sodium salicylate can be used as a chemical trap for hydroxyl radicals (OH•), the most damaging reactive oxygen species (ROS), yielding 2,5-dihydroxybenzoic acid), (2,5-DHBA) and 2,3-dihydroxybenzoic acid (2,3-DHBA). The measurement of these two acids in the plasma allows to indirectly assess the production of OH• radicals.
The aim of the study was to investigate the effect of hyaluronic acid (HYA) (30 mg/kg i.p.) or chondroitin-4-sulphate (C4S) (30 mg/kg i.p.), on free radical production in Lewis rats subjected to CIA.
After the immunization with bovine collagen type II in complete Freund's adjuvant, rats developed an erosive hind paw arthritis, that produced high plasma OH• levels assayed as 2,3-DHBA and 2,5-DHBA, primed lipid peroxidation, evaluated by analyzing conjugated dienes (CD) in the articular cartilage; decreased the concentration of endogenous vitamin E (VE) and catalase (CA) in the joint cartilage; enhanced macrophage inflammatory protein-2 (MIP-2) serum levels and increased elastase (ELA) evaluated as an index of activated leukocyte polymophonuclear (PMNs) accumulation in the articular joints.
The administration of HYA and C4S starting at the onset of arthritis (day 11) for 20 days, limited inflammation and the clinical signs in the knee and paw, reduced OH• production, decreased CD levels, partially restored the endogenous antioxidants VE and CA, reduced MIP-2 serum levels and limited PMNs infiltration.
The results indicate that the GAGs HYA and C4S significantly reduce free radical production in CIA and could be used as a tool to investigate the role of antioxidants in RA.
Document Type: Research Article
Affiliations: 1: Department of Biochemical, Physiological and Nutritional Sciences, School of Med., University of Messina, Policlinico Universitario, Torre Biologica, 5° piano, I-98125- Messina, Italy 2: Department of Morphology, Biochem., Physiology and Animal Production, School of Veterinary Med., University of Messina, Contrada Annunziata, 98168- Messina, Italy 3: Department of Experimental and Clini. Med. and Pharmacology, School of Medicine, University of Messina, Policlinico Universitario, Torre Biologica, 5° piano, 98125- Messina, Italy 4: Haematologic Operative Unit, School of Medicine, University of Messina, Policlinico Universitario, PAD. E 98125- Messina, Italy
Publication date: 2003-01-01