Effect of redox-active drugs on superoxide generation from nitric oxide synthases: Biological and toxicological implications
Source: Free Radical Research, Volume 31, Number 6, 1999 , pp. 607-617(11)
Publisher: Informa Healthcare
In this article, we address the mechanism of superoxide formation from constitutive nitric oxide synthases (NOS). Merits and drawbacks of the various superoxide detection assays are reviewed. One of the most viable techniques for measuring superoxide from NOS is electron spin resonance (ESR) spin-trapping using a novel phosphorylated spin trap. Implications of superoxide and peroxynitrite formation from NOS enzymes in cardiovascular and cerebrovascular disorders are discussed.
Document Type: Research Article
Affiliations: 1: Department of Pathology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA 2: Biophysics Research Institute, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA 3: Health Sciences Center, University of Texas-San Antonio 4: Laboratoire Structure et RÃ©activiÃ© des EspÃ©ces ParamagnÃ©tiques, UniversitÃ© de Provence, Marseille, France
Publication date: January 1, 1999