Vascular non-protein thiols: Prooxidants or antioxidants in atherogenesis?
Source: Free Radical Research, Volume 31, Number 6, 1999 , pp. 487-491(5)
Publisher: Informa Healthcare
Abstract-cell-mediated lipoprotein oxidation may be due to generation of non-protein thiols (NP-SH) from cystine with formation of oxidizing species. However, NP-SH, especially GSH, may also exert antioxidant effects in vitro and in vivo. To further investigate whether vascular NP-SH could be prooxidants or antioxidants in atherosclerosis, we have correlated the aortic content of NP-SH with that of lipoperoxides in 10 rabbits fed on a fat-enriched atherogenic diet for 9 weeks. As compared to 7 control rabbits, aortic NP-SH and lipoperoxides were significantly increased in the fat-fed animals. The levels of NP-SH were strongly and inversely correlated with those of lipid peroxidation in the atherosclerotic aorta (rs -0.92, P<0.0001 for thiobarbituric acid reactive substances, and rs-0.80, P<0.01 for fluorescent damage products of lipid peroxidation). A similar trend was evident also in the control rabbits (rs -0.60 for both indices of lipid peroxidation). Thus, the present data suggest that vascular NP-SH exert significant antioxidant-antilipoperoxidative effects in vivo especially in fat diet-related atherogenic conditions.
Document Type: Research Article
Affiliations: 1: Dipartimento di Medicina e Scienze dell'Invecchiamento, Universita' 'G. d'Annunzio', Facolta' di Medicina e Chirurgia, 66100, Chieti, Italy 2: Istituto di Scienze Biochimiche, Universita' 'G. d'Annunzio', Facolta' di Medicina e Chirurgia, 66100, Chieti, Italy
Publication date: January 1, 1999