Protein glycation inhibitory activities of Lawsonia inermis and its active principles

Authors: Sultana, Nighat¹; Choudhary, Muhammed Iqbal²; Khan, Ambrin²

Source: Journal of Enzyme Inhibition and Medicinal Chemistry, Volume 24, Number 1, February 2009 , pp. 257-261(5)

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Abstract:

The protein glycation inhibitory activity of ethanolic extract of Lawsonia inermis (henna) plant tissues was evaluated in vitro using the model system of bovine serum albumin and glucose. Protein oxidation and glycation are posttranslational modifications that are implicated in the pathological development of many age-related disease processes. This study investigated the effects of Lawsonia inermis ethanolic extract and its components, on protein damage induced by a free radical generator in in vitro assay system. We found that alcoholic extract of Lawsonia inermis can effectively protect against protein damage and showed that its action is mainly due to Lawsone. In addition, the presence of gallic acid also plays an important role in the protective activity against protein oxidation and glycation. Two known compounds, namely, Lawsone and gallic acid previously isolated from this plant were subjected to glycation bioassay for the first time. It was found that the alcoholic extract, lawsone (1) and gallic acid (2) showed significant inhibition of Advanced Glycated End Products (AGEs) formation and exhibit 77.95%, 79.10% and 66.98% inhibition at a concentration of 1500 μg/mL, 1000 μg/mL and 1000 μM respectively. Lawsonia inermis, compounds 1 and 2 were found to be glycation inhibitors with IC50 82.06 ± 0.13 μg/mL, 67.42 ± 1.46 μM and 401.7 ± 6. 23 μM respectively. This is the first report on the glycation activity of these compounds and alcoholic extract of Lawsonia inermis.

Keywords: Lawsonia inermis; lawsone; gallic acid; antioxidant; glycation, inhibitors

Document Type: Research article

DOI: http://dx.doi.org/10.1080/14756360802057500

Affiliations: 1: PCSIR Laboratories Complex, Pharmaceutical Research Center, Karachi, Pakistan 2: International Center for Chemical Sciences, H. E. J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan

Publication date: 2009-02-01

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Protein glycation inhibitory activities of Lawsonia inermis and its active principles

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