Authors: Bogusiewicz, Michał¹; Stryjecka-Zimmer, Marta²; Postawski, Krzysztof¹; Jakimiuk, Artur³; Rechberger, Tomasz¹

Source: Gynecological Endocrinology, Volume 23, Number 9, September 2007 , pp. 541-546(6)

Publisher: Informa Healthcare

Abstract:

Background and aim. Matrix metalloproteinase-2 and -9 (MMP-2 and -9) are proteolytic enzymes degrading extracellular matrix proteins, mainly collagen type IV. Recent reports show that these proteases may be implicated in the growth of uterine leiomyoma. The aim of the present study was to evaluate the activity of MMP-2 and MMP-9, the contents of their tissue inhibitors (TIMP-1 and TIMP-2) and the immunolocalization of collagen type IV in uterine leiomyoma and corresponding myometrium. Materials and methods. Material for the study comprised specimens of uterine leiomyomas and corresponding myometrium derived from 20 hysterectomized women. The activity of MMP-2 and MMP-9 in tissue extracts was evaluated by semi-quantitative zymography. TIMPs were measured by enzyme-linked inmmunosorbent assay. Protein immunohistochemistry was applied for detection of collagen type IV. Results. Activity and activation ratio of MMP-2 were significantly higher in leiomyomas than myometrium. The activity of MMP-9 was weak and did not differ between the investigated tissues. Contents of TIPM-1 and TIPM-2 were similar in both tissues. In both leiomyomas and myometrium, collagen type IV was localized in the extracellular matrix embedding bundles of smooth muscle cells, but was absent in areas of extracellular matrix accumulation within leiomyomas and in larger septa separating muscle fibers in normal myometrium. Conclusion. MMP-2 may be implicated in pathogenesis of leiomyoma.

Keywords: Uterine leiomyoma; metalloproteinase; collagen

Document Type: Research article

DOI: http://dx.doi.org/10.1080/09513590701557416

Affiliations: 1: Second Department of Gynecology, Medical University of Lublin, Lubin, Poland 2: Department of Biochemistry and Molecular Biology, Medical University of Lublin, Lubin, Poland 3: Second Department of Gynecology, Medical University of Lublin, Lubin, Poland,Polish Academy of Science, Medical Research Centre, Warsaw, Poland

Publication date: 2007-09-01

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