A study of the prevalence of hydrogen peroxide generating Lactobacilli in bacterial vaginosis: the determination of H2O2 concentrations generated, in vitro , by isolated strains and the levels found in vaginal secretions of women with and without infection
Authors: Al-Mushrif S.; Jones B. M.
Source: Journal of Obstetrics & Gynaecology, Volume 18, Number 1, 1 January 1998 , pp. 63-67(5)
Publisher: Informa Healthcare
Abstract:The concentration of hydrogen peroxide in the vaginal secretions of normal women were compared with levels found in women infected with bacterial vaginosis (BV). The mean concentration found in 90 normal samples was 0.171 mug per ml, whereas in women with BV, the mean level was 0.04 mug per ml ( P 0.001). In vitro culture experiments using TMB agar showed that in normal women, 75% of Lactobacillus strains produced H O and 2 2 25% did not, whereas in women "infected" with BV. 14% of the isolates produced H O and 86% did not ( P 0.001). 2 2 Quantitative culture tests showed that both anaerobic and facultative strains of Lactobacilli isolated from normal women generated significantly higher levels of H O than 2 2 strains cultured from women with BV. Anaerobic strains isolated from normal women produced a mean level of 6.8 mug per ml, compared with the mean level of 1.4 mug per ml from women with BV. Facultative strains from normal women produced mean levels of 18.3 mug per ml compared with 2.4 mug per ml produced by strains from BV-infected women, which had been incubated under the same conditions ( P 0.001). There results indicate that Lactobacilli which produce H O probably have a significant role in 2 2 protecting the vaginal ecosystem from BV infection.
Document Type: Research article
Publication date: 1998-01-01