Evaluation of RGD Modification on Collagen Matrix

Authors: Ren, Dongfeng¹; Hou, Shuxun¹; Wang, Huadong¹; Luo, Dianzhong¹; Zhang, Lihai²

Source: Artificial Cells, Blood Substitutes, and Biotechnology (formerly known as Artif, Volume 34, Number 3, May-June 2006 , pp. 293-303(11)

Publisher: Informa Healthcare

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Abstract:

Objective: To observe the changes during the process of modificating collagen with Arg-Gly-Asp (RGD) peptide, a series experiments were designed. Methods: The 3D porous collagen matrices modified with RGD peptide were constructed by lyophilization. The modified and unmodified matrices were characterized by Scanning Electron Microscopy (SEM) and Electron Spectroscopy for Chemical Analysis (ESCA). Fibroblasts were used to evaluate the cell compatibility of the matrices. Results: In terms of cell growth, the cells attached much better on the modified matrix than on the unmodified one. Compare to the unmodified matrices, the polar groups on the modified matrix increased. Conclusions: The introducing of specific RGD receptor-mediated adhesion site on matrices obviously enhanced the cells adhesion on collagen matrices.

Keywords: Collagen matrix; RGD; Surface modification; Periodate oxidation

Document Type: Research article

DOI: http://dx.doi.org/10.1080/10731190600683829

Affiliations: 1: Department of Orthopaedics, Institute of Orthopaedics, Beijing, P.R. China 2: Department of Stomatology, Chinese PLA General Hospital, Beijing, P.R. China

Publication date: 2006-05-01

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