Proteome-Scale Analysis of Biochemical Activity

Authors: Phizicky, Eric; Grayhack, Elizabeth

Source: Critical Reviews in Biochemistry and Molecular Biology, Volume 41, Number 5, September-October 2006 , pp. 315-327(13)

Publisher: Informa Healthcare

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Abstract:

During the last 10 years, there has been a large increase in the number of genome sequences available for study, altering the way that the biology of organisms is studied. In particular, scientific attention has increasingly focused on the proteome, and specifically on the role of all the proteins encoded by the genome. We focus here on several aspects of this problem. We describe several technologies in widespread use to clone genes on a genome-wide scale, and to express and purify the proteins encoded by these genes. We also describe a number of methods that have been developed to analyze various biochemical properties of the proteins, with attention to the methodology and the limitations of the approaches, followed by a look at possible developments in the next decade.

Keywords: functional genomics; biochemical genomics; protein microarrays; high throughput cloning; enzyme genomics

Document Type: Research article

DOI: http://dx.doi.org/10.1080/10409230600872872

Affiliations: 1: Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, NY, USA

Publication date: 2006-09-01

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