Effect of ceramide analogs on interleukin-1α-induced production of prostaglandin E2 by amnion-derived (WISH) cells
Authors: Nakamura, Satomi; Kawano, Yasushi; Kamihigashi, Shoko; Sugano, Terumasa; Narahara, Hisashi; Miyakawa, Isao
Source: Acta Obstetricia et Gynecologica Scandinavica, Volume 80, Number 1, January 2001 , pp. 12-17(6)
Publisher: Informa Healthcare
Prostaglandin E2 ( PGE2 ) is reportedly synthesized in the amnion, and its levels are increased during labor. Our objective was to measure the level of PGE2 induced by interleukin (IL)-1α following treatment with ceramide analogs in amnion-derived cells. Methods.
Amnion-derived (WISH) cells were cultured and stimulated by IL-1α, IL-1 receptor antagonist (ra), C2-ceramide and C6-ceramide. The levels of PGE2 in the media were measured by ELISA. The induction of prostaglandin H synthase ( PGHS)-2 mRNA was detected by reverse transcription and polymerase chain reaction ( RT-PCR). Results.
Following stimulation with IL-1α, the production of PGE2 could not be detected until incubation had continued for 2 h, but this production appeared to continue after 4 h of incubation. The production of PGE2 was significantly increased by IL-1α, and was suppressed by IL-1 ra, in a dose-dependent manner. PGE2 production was significantly increased by IL-1α and C2-ceramide as compared with IL-1α alone. However, PGE2 production was not significantly increased by IL-1α and C6-ceramide as compared with IL-1α alone. PGHS-2 mRNA were induced by treatment with IL-1α, and were strongly induced by treatment with IL-1α and C2-ceramide by RT-PCR. Conclusions.
Results suggest that IL-1α induce the PGHS-2 mRNA and stimulate the production of PGE2 by a mechanism that involves the sphingomyelin-ceramide system. Ceramide may be important in increasing the production of PGE2 during parturition.
Document Type: Research Article
Publication date: January 1, 2001