Mechanism of Photokilling LoVo Cells by Nano TiO2 Photocatalytic Oxidation Combined with Immunity and Electroporation Method
The nano TiO2 photocatalytic oxidation method combined with immunity and electroporation techniques performed high efficiency for photokilling LoVo cancer cells even at very low concentration (3.12 μg·mL-1). In this method, the LoVo cells could be killed completely within 30 min under the irradiation of UV light (wavelength 253.7 nm, 4 mW·cm-2). The mechanism of this process was investigated using confocal florescent microscopy, TEM, and comet assay methods. The results demonstrated that the antibody modified nano TiO2 particles could adsorb on the cell membrane, after electroporation it could enter the inside of LoVo cells and located around the karyon. Under the irradiation of UV light, the cell’s structure, organelle, and DNA were destroyed seriously, which caused the necroses of LoVo cells. Because the photocatalysis effect of nano TiO2 took place inside of cells, so the photokilling efficiency enhanced strongly.
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Document Type: Research Article
Publication date: 15 August 2008
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- Acta Physico-Chimica Sinica, founded in 1985, is sponsored by the Chinese Chemical Society and organized by the College of Chemistry and Molecular Engineering, PekingUniversity. Since 1997, Acta Physico-Chimica Sinica has been indexed in SCI of ISI (US). Acta Physico-Chimica Sinica is devoted to reporting new and original experimental and theoretical basic research of interest to physical chemists, biophysical chemists, and material physical chemists. Manuscripts that are essentially reporting data, applications of data, or reviews of the literature are not suitable for publication in Acta Physico-Chimica Sinica.
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