A Functional NF-B Binding Site in the Human Papillomavirus Type 16 Long Control Region

Authors: Fontaine V.; van der Meijden E.; de Graaf J.; ter Schegget J.; Struyk L.

Source: Virology, Volume 272, Number 1, June 2000 , pp. 40-49(10)

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Abstract:

By computer search, we identified one potential NF- kappa B binding site in the HPV16 long control region (LCR) at position 7554–7563 having two mismatches in comparison to the consensus NF- kappa B binding site of the Ig kappa L promoter. Bandshift experiments with nuclear extracts from HeLa cells or purified glutathione S-transferase–p65 fusion protein clearly demonstrated that NF- kappa B is able to bind to this region of the LCR. However, in comparison to NF- kappa B binding on a consensus probe, the affinity of NF- kappa B for this site is about 250-fold reduced. When mutations were introduced into this NF- kappa B binding site, the activity of the LCR was increased, strongly suggesting that NF- kappa B was acting as a transcriptional repressor in the context of the HPV16 LCR. In addition, overexpression of NF- kappa B p65 repressed the activity of the HPV16 LCR, strengthening this conclusion. Copyright 2000 Academic Press.

Language: English

Document Type: Research article

Affiliations: Department of Virology, University of Amsterdam, Meibergdreef 15, Amsterdam, 1105 AZ, The Netherlands

Publication date: 2000-06-01