Crystallization and preliminary X-ray diffraction studies of cobra venom beta-nerve growth factor

Authors: Gu L.1; Shen Y.1; Xu S.1; Shu Y.2; Jiang T.1; Lin Z.1

Source: Biochemical and Biophysical Research Communications, Volume 297, Number 4, October 2002 , pp. 1008-1010(3)

Publisher: Academic Press

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Abstract:

beta-Nerve growth factor (beta-NGF) is a 118 amino acid residue polypeptide with an important role in the survival and development of certain neuronal populations. Abeta-NGF purified from cobra venom was crystallized by the hanging-drop vapor diffusion method. The crystals belong to the tetragonal space group P41212 (or its enantiomorph P43212) with unit-cell parameters a=b=61.75, c=154.40Aring. X-ray data from these crystals were collected to 3.2 Aring resolution. Analysis of the packing density shows that the asymmetric unit probably contains two molecules. The self-rotation calculation implied that abeta-NGF dimer might exist in the asymmetric unit.

© 2002 Elsevier Science (USA)

Language: English

Document Type: Research article

DOI: 10.1016/S0006-291X(02)02323-9

Affiliations: 1: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 2: Guangxi Medical University, Institute of Snake Venom, Nanning 530021, China

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