Crystallization and preliminary X-ray diffraction studies of cobra venom -nerve growth factor

Authors: Gu L.¹; Shen Y.¹; Xu S.¹; Shu Y.²; Jiang T.¹; Lin Z.¹

Source: Biochemical and Biophysical Research Communications, Volume 297, Number 4, October 2002 , pp. 1008-1010(3)

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Abstract:

-Nerve growth factor (-NGF) is a 118 amino acid residue polypeptide with an important role in the survival and development of certain neuronal populations. A-NGF purified from cobra venom was crystallized by the hanging-drop vapor diffusion method. The crystals belong to the tetragonal space group P4₁2₁2 (or its enantiomorph P4₃2₁2) with unit-cell parameters a=b=61.75, c=154.40A ring . X-ray data from these crystals were collected to 3.2 A ring resolution. Analysis of the packing density shows that the asymmetric unit probably contains two molecules. The self-rotation calculation implied that a-NGF dimer might exist in the asymmetric unit.

Language: English

Document Type: Research article

DOI: http://dx.doi.org/10.1016/S0006-291X(02)02323-9

Affiliations: 1: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 2: Guangxi Medical University, Institute of Snake Venom, Nanning 530021, China

Publication date: 2002-10-01