Dynamics of Ca2+/calmodulin-dependent protein kinase II following acute myocardial ischemiatranslocation and autophosphorylation
Authors: Uemura A.1; Naito Y.2; Matsubara T.1
Source: Biochemical and Biophysical Research Communications, Volume 297, Number 4, October 2002 , pp. 997-1002(6)
Publisher: Academic Press
Abstract:
Ca2+/calmodulin-dependent protein kinase (CaMK) family is responsive to changes in the intracellularCa2+ concentration. However, their functions have not been well established in the ischemia/reperfusion heart. The effects of myocardial ischemia on CaMKII, the most strongly expressed form, were investigated using isolated rat hearts. Rat hearts were rendered globally ischemic by stopping perfusion for 15 min, and then reperfused, heart ventricles being analyzed in each phase. Western blotting detected a decrease in the cytosolic and concomitant increase in the particulate fraction of CaMKII following transient ischemia. Redistribution to the cytosol was revealed on reperfusion. Northern blot showed CaMKII gene expression decreased by ischemia. Furthermore, autoradiography and confocal immunohistochemical findings provided autophosphorylation of CaMKII in the cytosol, ischemia causing decrease, with gradual recovery on reperfusion. These results indicate a transient partial translocation of CaMKII accompanied by kinase activity, with residual myocardial CaMKII undergoing autophosphorylation during ischemia and reperfusion, demonstrating two different characteristic dynamics of CaMKII.
© 2002 Elsevier Science (USA)
Language: English
Document Type: Research article
DOI: http://dx.doi.org/10.1016/S0006-291X(02)02279-9
Affiliations: 1: Third Department of Internal Medicine, School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan 2: Department of Pharmacology, Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan
Publication date: 2002-10-01
- In this: publication
- By this: publisher
- In this Subject: Anatomy & Physiology , Biology , Biochemistry
- By this author: Uemura A. ; Naito Y. ; Matsubara T.

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