Insulin Resistance and Type 2 Diabetes Are Not Related to Resistin Expression in Human Fat Cells or Skeletal Muscle
Authors: Nagaev I.; Smith U.
Source: Biochemical and Biophysical Research Communications, Volume 285, Number 2, July 2001 , pp. 561-564(4)
Publisher: Academic Press
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Abstract:
Resistin is secreted by rodent fat cells and was recently postulated to be an important link between obesity and insulin resistance. We examined Resistin gene expression with real-time RT-PCR in human isolated fat cells, adipose tissue, and muscle from 42 individuals of varying degrees of overweight and who had normal insulin sensitivity or were insulin-resistant or Type 2 diabetic. Resistin was not expressed in human muscle nor was it expressed in most human isolated fat cells or intact biopsies. No difference was found between normal, insulin-resistant, or Type 2 diabetic samples. However, a very low but specific Resistin expression could be demonstrated in isolated fat cells and intact adipose tissue from some individuals (n = 3 and n = 4, respectively). There was no evidence for the expression of splice variants in the human samples. Thus, Resistin does not seem to be an important link to insulin resistance and Type 2 diabetes in human. Copyright 2001 Academic Press.
Keywords: resistin; adipose tissue; muscle; insulin resistance; Type 2 diabetes; gene expression
Language: English
Document Type: Research article
Affiliations: Department of Internal Medicine, Sahlgrenska University Hospital, Göteborg, S-413 45, Sweden:
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