IngentaConnect Diversity of Voltage-Gated Sodium Channels in the Ascidian Larval...

Authors: Nagahora H.¹; Okada T.¹; Yahagi N.³; Chong J.A.²; Mandel G.²; Okamura Y.^{1, 4}

Source: Biochemical and Biophysical Research Communications, Volume 275, Number 2, August 2000 , pp. 558-564(7)

Abstract:

To gain insight into the origin of the molecular diversity of voltage-gated sodium channels (NaVs), a putative sodium channel gene (TuNa2) was cloned from the protochordate ascidian. TuNa2 showed two unusual features in its primary structure; (1) lysine in the P-region of the third repeat, a critical site determining ion selectivity, was changed to glutamic acid, predicting that the ionic permeability would not be rigidly sodium-selective (2) the III-IV linker, determinant of fast inactivation, was only weakly conserved. In contrast with a pan-neuronally expressed NaV (TuNa1), expression of TuNa2 was confined to subsets of neurons including motor neurons, suggesting that TuNa2 plays specialized roles in electrical activities unique to these neurons. Basic FGF, a neural inducer in the ascidian embryo, induces TuNa2 RNA expression in the ectodermal cells at lower doses than that required for TuNa1 gene expression. Thus, two types of NaV may play distinct roles and their gene expressions are controlled by distinct mechanisms. Copyright 2000 Academic Press.

Keywords: sodium channel; ascidian; bFGF; ion selectivity; motor neuron

Language: English

Document Type: Research article

Affiliations: 1: Ion Channel Group, AIST, Higashi 1-1, Ibaraki, Tsukuba, 305, Japan 2: Department of Neurobiology and Behavior, State University of New York at Stony Brook, Stony Brook, New York, 11794 3: Department of Metabolic Diseases, University of Tokyo, Hongo 7-3-1, Tokyo, 113, Japan 4: Graduate School of Interdisciplinary Sciences, University of Tokyo, Meguro, Japan

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$54.38 plus tax Refund Policy

Diversity of Voltage-Gated Sodium Channels in the Ascidian Larval Nervous System