Purification, Characterization, and cDNA Cloning of a New Fibrinogenlytic Venom Protein, Agkisacutacin, from Agkistrodon acutus Venom
Authors: Cheng X.1, 2; Qian Y.1; Liu Q.1; Li B.X.Y.2; Zhang M.1; Liu J.1
Source: Biochemical and Biophysical Research Communications, Volume 265, Number 2, November 1999 , pp. 530-535(6)
Publisher: Academic Press
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Abstract:
Agkisacutacin is a new fibrinogenlytic protein from Agkistrodon acutus venom. It consists of two heterologous subunits linked by an intersubunit disulfide bond. The cDNAs encoding the two chains of Agkisacutacin were cloned from a 
gt11 cDNA library of the snake venom gland and sequenced, including the leader peptides (23/23 amino acid residues) and mature subunits (129/123 amino acid residues). It is structurally related to the family of IX/X-binding protein (IX/X-bp)-like proteins and shows high similarity (
-70%/
-64%) to habu IX/X-bp from Trimeresurus flavoridis, but displays distinct biological activity with direct action on fibrinogen. Copyright 1999 Academic Press.
Language: English
Document Type: Research article
Affiliations: 1: School of Life Science, University of Science and Technology of China, Hefei, Anhui, 230027, People's Republic of China 2: Siu-Fung USTC Pharmaceutical Company, R&D, People's Republic of China
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