Purification, Characterization, and cDNA Cloning of a New Fibrinogenlytic Venom Protein, Agkisacutacin, from Agkistrodon acutus Venom

Authors: Cheng X.^{1, 2}; Qian Y.¹; Liu Q.¹; Li B.X.Y.²; Zhang M.¹; Liu J.¹

Source: Biochemical and Biophysical Research Communications, Volume 265, Number 2, November 1999 , pp. 530-535(6)

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Abstract:

Agkisacutacin is a new fibrinogenlytic protein from Agkistrodon acutus venom. It consists of two heterologous subunits linked by an intersubunit disulfide bond. The cDNAs encoding the two chains of Agkisacutacin were cloned from a lambda gt11 cDNA library of the snake venom gland and sequenced, including the leader peptides (23/23 amino acid residues) and mature subunits (129/123 amino acid residues). It is structurally related to the family of IX/X-binding protein (IX/X-bp)-like proteins and shows high similarity ( alpha -70%/ beta -64%) to habu IX/X-bp from Trimeresurus flavoridis, but displays distinct biological activity with direct action on fibrinogen. Copyright 1999 Academic Press.

Language: English

Document Type: Research article

Affiliations: 1: School of Life Science, University of Science and Technology of China, Hefei, Anhui, 230027, People's Republic of China 2: Siu-Fung USTC Pharmaceutical Company, R&D, People's Republic of China

Publication date: 1999-11-01