Induction of Hsp70 in the Fungus Rhizopus nigricans

Authors: Ccaron ernila B.; Ccaron re scaron nar B.; Breskvar K.

Source: Biochemical and Biophysical Research Communications, Volume 265, Number 2, November 1999 , pp. 494-498(5)

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Abstract:

We characterised two positive clones, RnH20/2 and RnH2/3, isolated from the cDNA library prepared from the fungus Rhizopus nigricans previously exposed to heat shock. Nucleotide sequences of both cDNA clones contained open reading frames for polypeptides with a significant amino acid identity to each other and to cytoplasmic members of Hsp70s from different eukaryotic organisms. Northern blot analysis, using a fragment of RnH2/3 as a probe, revealed that in the fungus R. nigricans the Hsp70 transcripts were inducible with deoxycorticosterone and testosterone as well as with heat stress, ethanol, CuSO₄, and H₂O₂. This is the first report on isolation of cDNAs encoding Hsp70s from the fungal phylum Zygomycota. Copyright 1999 Academic Press.

Language: English

Document Type: Research article

Affiliations: Faculty of Medicine, University of Ljubljana, Vrazov trg 2, Slovenia

Publication date: 1999-11-01