In Vivo Gene Transfer of an Extracellular Domain of Platelet-Derived Growth Factor Receptor by the HVJ-Liposome Method Ameliorates Bleomycin-Induced Pulmonary Fibrosis

Authors: Yoshida M.¹; Sakuma-Mochizuki J.¹; Abe K.¹; Arai T.¹; Mori M.¹; Goya S.¹; Matsuoka H.¹; Hayashi S.¹; Kaneda Y.²; Kishimoto T.¹

Source: Biochemical and Biophysical Research Communications, Volume 265, Number 2, November 1999 , pp. 503-508(6)

Publisher: Academic Press

Buy & download fulltext article:

Price: $52.63 plus tax (Refund Policy)

Abstract:

A number of investigators have reported augmented expression of PDGF in lungs with idiopathic pulmonary fibrosis (IPF) or with other types of pulmonary fibrosis. To accomplish such a regulation of PDGF activity, we constructed an expression plasmid of the extracellular domain of PDGF receptor beta chain (XR), which lacks intracellular tyrosine kinase domain and transmembrane portions, and estimated the therapeutic effects of XR gene transfer through the trachea on bleomycin-induced lung fibrosis of C57BL/6 mice using the hemagglutinating virus of Japan(HVJ)-liposome method. The XR gene transfer ameliorated the increases in the wet weight and hydroxyproline content and the histopathologic changes of the lung induced by bleomycin. These findings suggest that PDGF plays a crucial role in the pathogenesis of pulmonary fibrosis, and that XR gene transfer using the HVJ-liposome method may limit the progression of pulmonary fibrosis. Copyright 1999 Academic Press.

Language: English

Document Type: Research article

Affiliations: 1: Department of Molecular Medicine 2: Department of Molecular Medicine, Division of Gene Therapy Science, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan

Publication date: 1999-11-01