Quantification of the cAMP Response Element Binding Protein in Ventricular Nuclear Protein from Failing and Nonfailing Human Hearts
Authors: Müller F.U.1; Bokník P.1; Knapp J.1; Lüss H.1; Neumann J.1; Vahlensieck U.1; Böhm M.2; Deng M.C.3; Scheld H.H.3; Schmitz W.1
Source: Biochemical and Biophysical Research Communications, Volume 236, Number 2, July 1997 , pp. 351-354(4)
Publisher: Academic Press
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Abstract:
Alterations in the expression of myocardial regulatory proteins (e.g. 
-adrenoceptor, inhibitory G-proteins) in human heart failure are associated with excessive stimulation of the cAMP signalling pathway by endogenous catecholamines. The transcription factor cAMP response element binding protein (CREB) mediates cAMP-dependent transcriptional activation and is expressed in the human heart. Here, CREB protein was immunologically quantified in ventricular nuclear protein preparations from nonfailing donor hearts (n = 8) and from failing hearts transplanted due to dilative (n = 10) or ischemic cardiomyopathy (n = 6). CREB expression was unchanged in ventricular nuclei from failing hearts compared to the nonfailing controls suggesting that expressional alterations in human heart failure cannot be explained by altered expression of CREB.
Language: English
Document Type: Research article
Affiliations: 1: Institut für Pharmakologie und Toxikologie, Universität Münster, Munster, Germany 2: Klinik III für Innere Medizin, Universität Köln, Cologne, Germany 3: Klinik und Poliklinik für Thorax-, Herz-, und Gefäßchirurgie, Universität Münster, Munster, Germany
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