Early Cytoplasmic Acidification in Retinamide-Mediated Apoptosis of Human Promyelocytic Leukemia Cells

Authors: Angoli D.¹; Delia D.²; Wanke E.¹

Source: Biochemical and Biophysical Research Communications, Volume 229, Number 2, December 1996 , pp. 681-685(5)

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Abstract:

The retinamide-mediated apoptosis of promyelocytic cells was investigated using pH i - and DNA-sensitive fluorescent probes (BCECF and Hoechst 33342). Acidification and apoptosis were observed during prolonged (0-12 h) exposure to retinamide (1 muM), but were absent in a retinamide-resistant clone . The analysis of experiments performed by simultaneous staining with the two dyes showed that acidification and apoptosis are correlated; the half-time for acidification is about 5 h while that for apoptosis is 80% longer. On the whole, these results suggest that apoptosis is preceded by an early mechanism of acidification in human leukemia cells treated by a retinoid of clinical interest in cancer chemo prevention and therapy.

Language: English

Document Type: Research article

Affiliations: 1: Laboratory of Electrophysiology, University of Milano, Via Celoria 26, Milano, Italy 2: Division of Experimental Oncology A, Istituto Nazionale Tumori, Via Venezian, 1, Milano, I-20133, Italy

Publication date: 1996-12-01