Crosslinking of SRF to the c- fos SRE CArG Box Guanines Using Photo-active Thioguanine Oligodeoxynucleotides
Authors: Cahill M.A.1; Nordheim A.1; Xu Y.Z.2
Source: Biochemical and Biophysical Research Communications, Volume 229, Number 1, December 1996 , pp. 170-175(6)
Publisher: Academic Press
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Abstract:
6-Thioguanine (thioG) was chemically incorporated into 25-base oligodeoxynucleotides encoding the c- fos serum response element (SRE) at positions corresponding to each guanine of the CArG box, which only slightly impaired DNA binding by the Serum Response Factor (SRF). Upon exposure to long wavelength UV light each thioG-containing SRE could be crosslinked to SRF, with efficiencies ranging from <1 to 25% of the complex depending on the position of thioG in the SRE and on the UV source used. Crosslinking was strongest to the 3\' side of the CArG box, and to the outer rather than the inner CArG box guanines, consistent with hydrogen bonds formed between SRF and the outer guanines in the crystal structure [Pellegrini et al., Nature 376, 490, 1995]. The crosslinked product was found to be chemically unstable. Possible mechanisms of crosslink formation are discussed.
Language: English
Document Type: Research article
Affiliations: 1: Hannover Medical School, Institute for Molecular Biology, Konstanty-Gutschow-Strasse 8, Hannover, 30625, Germany 2: Department of Biochemistry and Molecular Biology, University College London, Gower Street, London, WC1E 6BT, England
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