Crosslinking of SRF to the c- fos SRE CArG Box Guanines Using Photo-active Thioguanine Oligodeoxynucleotides

Authors: Cahill M.A.1; Nordheim A.1; Xu Y.Z.2

Source: Biochemical and Biophysical Research Communications, Volume 229, Number 1, December 1996 , pp. 170-175(6)

Publisher: Academic Press

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content

Abstract:

6-Thioguanine (thioG) was chemically incorporated into 25-base oligodeoxynucleotides encoding the c- fos serum response element (SRE) at positions corresponding to each guanine of the CArG box, which only slightly impaired DNA binding by the Serum Response Factor (SRF). Upon exposure to long wavelength UV light each thioG-containing SRE could be crosslinked to SRF, with efficiencies ranging from <1 to 25% of the complex depending on the position of thioG in the SRE and on the UV source used. Crosslinking was strongest to the 3\' side of the CArG box, and to the outer rather than the inner CArG box guanines, consistent with hydrogen bonds formed between SRF and the outer guanines in the crystal structure [Pellegrini et al., Nature 376, 490, 1995]. The crosslinked product was found to be chemically unstable. Possible mechanisms of crosslink formation are discussed.

Language: English

Document Type: Research article

Affiliations: 1: Hannover Medical School, Institute for Molecular Biology, Konstanty-Gutschow-Strasse 8, Hannover, 30625, Germany 2: Department of Biochemistry and Molecular Biology, University College London, Gower Street, London, WC1E 6BT, England

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$54.38 plus tax      Refund Policy

 

OR

Back to top

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages.
Page Help Click here for Page Help
Shopping cart
Tools
Sign in






Need to register?
Sign up here
Text size: A | A | A | A