2,3-Butanedione 2-Monoxime (BDM) Induces Calcium Release from Canine Cardiac Sarcoplasmic Reticulum

Authors: Phillips R.M.; Altschuld R.A.

Source: Biochemical and Biophysical Research Communications, Volume 229, Number 1, December 1996 , pp. 154-157(4)

Publisher: Academic Press

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Abstract:

2,3-Butanedione 2-monoxime (BDM) is a well known inhibitor of skeletal and cardiac muscle contraction. Recently, it has been discovered that BDM has an influence on the sarcoplasmic reticulum (SR). We investigated the effects of BDM on the SR in our digitonin lysed myocyte system, which measures accumulated SR Ca 2+ . While BDM (30 mM) had no effect on SR Ca 2+ uptake (under conditions that included Ca 2+ release channel efflux inhibitors), it induced SR Ca 2+ release (no efflux inhibitors) with a maximal reduction of 72% of SR Ca 2+ at pCa 6.0. A titration showed that even 5 mM BDM resulted in a 45% reduction at that same pCa. Also, a positive correlation was found between the degree of BDM induced Ca 2+ release and free Ca 2+ concentration. Thus, the use of even low concentrations of BDM as an excitation-contraction uncoupler must be approached with caution.

Language: English

Document Type: Research article

Affiliations: Department of Medical Biochemistry, The Ohio State University, Columbus, Ohio, 43210:

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