Molecular Cloning of a Novel Rat Salt-Tolerant Protein by Functional Complementation in Yeast

Authors: Tsuji E.¹; Tsuji Y.²; Misumi Y.³; Fujita A.⁴; Sasaguri M.¹; Ideishi M.¹; Arakawa K.¹

Source: Biochemical and Biophysical Research Communications, Volume 229, Number 1, December 1996 , pp. 134-138(5)

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Abstract:

To elucidate the genetic basis of salt-sensitivity in mammalian hypertension, we isolated six rat complementary DNAs by functional complementation in yeast. These genes were able to substitute for the salt-tolerant activity of HAL1 which confers salt tolerance by modulating the cation transport system in yeast. We identified these genes as beta-globin, lambda-crystallin, androgen-regulated protein, mitochondrial cytochrome b, a homologue of infant brain cDNA, and a novel gene, called salt-tolerant protein (STP). STP contains 1964 bp nucleotides and an open reading frame which encodes 496 amino acid residues. Northern blot analysis showed that STP mRNA is expressed in various rat tissues.

Language: English

Document Type: Research article

Affiliations: 1: 2nd Department of Internal Medicine, Fukuoka University School of Medicine, Jonan-ku, Fukuoka, 814-80, Japan 2: Departments of Urology, Fukuoka University School of Medicine, Jonan-ku, Fukuoka, 814-80, Japan 3: Departments of Biochemistry, Fukuoka University School of Medicine, Jonan-ku, Fukuoka, 814-80, Japan 4: National Institute of Bioscience and Human Technology, Agency of Industrial Science & Technology, Tsukuba, 305, Japan

Publication date: 1996-12-01