Expression of a Synthetic Gene Encoding the Sweet-Tasting Protein Thaumatin in Escherichia coli

Authors: Faus I.¹; Patino C.²; Rio J.L.¹; del C.²; Barroso H.S.¹; Rubio V.²

Source: Biochemical and Biophysical Research Communications, Volume 229, Number 1, December 1996 , pp. 121-127(7)

Publisher: Academic Press

Abstract:

A synthetic gene encoding the aminoacid sequence of the sweet-tasting protein thaumatin II has been assembled and expressed in Escherichia coli. Immunoblotting analysis shows that the expressed recombinant thaumatin has the same molecular weight as the protein from its natural source, the plant Thaumatoccocus daniellii Benth.

Language: English

Document Type: Research article

Affiliations: 1: Laboratory of Biotechnology, URQUIMA, S.A. (URIACH GROUP), Arnau de Vilanova, 22-42, Sant Fost de Campsentelles (Barcelona), 08105, Spain 2: Campus Canto Blanco, Centro Nacional de Biotecnologia (CSIC-UAM), Madrid, 28049, Spain

Publication date: 1996-12-01

• In this: publication
• By this: publisher
• In this Subject: Anatomy & Physiology ,  Biology ,  Biochemistry
• By this author: Faus I. ;  Patino C. ;  Rio J.L. ;  del C. ;  Barroso H.S. ;  Rubio V.

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