Temporal and Spatial Resolution of Ca 2+ Release and Influx in Human Neutrophils Using a Novel Confocal Laser Scanning Mode

Authors: Pettit E.J.; Hallett M.B.

Source: Biochemical and Biophysical Research Communications, Volume 229, Number 1, December 1996 , pp. 109-113(5)

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Abstract:

Confocal laser scanning demonstrated that stimulation of neutrophils with the surface receptor agonist, f-met-leu-phe, resulted in the release of stored Ca 2+ from a single site. From reconstructions of neutrophils stained with acridine orange, it was shown that the central Ca 2+ release site was always close to the nucleus, and correlated with a site which stained with DiOC(6) 3 . Elevated Ca 2+ was locally restricted to within 1 mum of this site. Release of Ca 2+ by this pathway was accompanied by the influx of Ca 2+ , less than 1 second after store release. In each neutrophil, the Ca 2+ store release component preceded the Ca 2+ influx, and their spatial separation suggested communication between the Ca 2+ store and the plasma membrane by a messenger. The (distance) 2 of the release site from the plasma membrane was correlated to the time between Ca 2+ release and influx, consistent with the diffusion of a messenger from the storage site signalling Ca 2+ influx.

Language: English

Document Type: Research article

Affiliations: Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff, CF4 4XN, United Kingdom

Publication date: 1996-12-01