Solamargine Purified from Solanum incanum Chinese Herb Triggers Gene Expression of Human TNFR I Which May Lead to Cell Apoptosis

Authors: Hsu S.H.^{1, 2}; Tsai T.R.²; Lin C.N.²; Yen M.H.²; Kuo K.W.¹

Source: Biochemical and Biophysical Research Communications, Volume 229, Number 1, December 1996 , pp. 1-5(5)

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Abstract:

Solamargine possessed a potent cytotoxicity to human hepatocyte (Hep3B) and normal skin fibroblast. The inhibition curves of solamargine to the both cells were essentially overlapped, suggesting a parallel effect for the cell death. To define mechanism of cytotoxicity of solamargine, the changes of morphology and DNA content in cells were studied. A sub-G 1 cell stage was drastically increased after 3-h incubation with solamargine. The results evidence that solamargine arises cell death by apoptosis. In addition, the gene expression of TNFR I were up-regulated within 30 min of solamargine treatment. Since TNF Receptor I has been involved in apoptosis, the overexpression of TNF receptor I may be related with the mechanism of cytotoxicity of solamargine. This communication is the first report that a component of Chinese herbs triggers gene expression of human TNFR I which may lead to cell apoptosis.

Language: English

Document Type: Research article

Affiliations: 1: Department of Biochemistry, Kaohsiung Medical College, Kaohsiung, 807, Taiwan 2: School of Pharmacy, Kaohsiung Medical College, Kaohsiung, 807, Taiwan

Publication date: 1996-12-01