The Contribution of Endogenous Polyamines to the Permeability Transition of Rat Liver Mitochondria

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Abstract:

Spermine and spermidine are present in rat liver mitochondria at the concentrations of 3.5 and 1.8 nanomoles/mg protein, respectively. Addition of 50 muM Ca 2+ and 1 mM Pi to respiring mitochondria induces, concomitantly with mitochondrial swelling and Mg 2+ efflux, a consistent release of polyamines, which is either prevented by cyclosporin A or Mg 2+ . Addition of 0.1 mM spermine to mitochondria deenergized by Ca 2+ and phosphate restores, like 1 mM Mg 2+ , transmembrane potential at the physiological level, while either cyclosporin A or ADP are ineffective. The presence of polyamines in the matrix space should be considered relevant, like Mg 2+ , in controlling the permeability transition of liver mitochondria.

Document Type: Research Article

Affiliations: Dipartimento di Chimica Biologica, Universita di Padova, Centro di studio delle Biomembrane del C.N.R. di Padova, via Trieste, 75, Padova, 35121, Italy

Publication date: September 1, 1996

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