Glucose Induces Cytoplasmic Na + Oscillations in Pancreatic beta-Cells

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The cytoplasmic Na + concentration ([Na + ] i ) was measured in individual mouse beta-cells and islet cell aggregates using the indicator SBFI. Small oscillations of [Na + ] i were occasionally observed after raising glucose from 3 to 11 mM. Distinct oscillations with a frequency of 0.29 ± 0.02 min -1 (n=18) and amplitudes of 7.4 ± 0.5 mM (n=18) were obtained when also adding the Na + channel agonist veratridine. The oscillations were counteracted by tetrodotoxin or by lowering the glucose concentration to 3 mM. Glucose failed to induce oscillations when increasing Na + entry by co-transport with L-glycine or raising [Na + ] i by blocking the Na/K pump with ouabain. The results indicate that glucose can induce oscillations of [Na + ] i in pancreatic beta-cells, although this effect is usually less obvious due to activation of an efficient Na/K pump.

Document Type: Research Article

Affiliations: Department of Medical Cell Biology, Uppsala University, Biomedicum, Uppsala, S-751 23, Sweden

Publication date: September 1, 1996

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