Proton Magnetic Resonance Studies of Methionine Enkephalin
Source: Biochemical and Biophysical Research Communications, Volume 226, Number 3, September 1996 , pp. 796-800(5)
Publisher: Academic Press
Abstract:In the literature there exist two conflicting pieces of data concerning the thermodynamics of the Cu 2+ -methionine enkephalin complex. One study utilized 1 H nuclear magnetic resonance (NMR) to study the solution phase Cu 2+ -enkephalin binding and reported a strong interaction over a wide pH range. Another study utilized potentiometry to measure a logbeta 101 of 4.6, a value representative of a weak metal-ligand complex. In order to resolve this discrepancy a 300 MHz 1 H NMR is used to study the protonation sequence of methionine enkaphalin, a bioactive peptide, and its complexation with seven diamagnetic metals (Ba 2+ , Ca 2+ , Zn 2+ , La 2+ , Mg 2+ , Sr 2+ and Cd 2+ ).
Document Type: Research Article
Publication date: September 1, 1996