Microheterogeneity of C-Reactive Protein in the Sera of Fish Labeo rohita Induced by Metal Pollutants

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Abstract:

A group of C-reactive proteins (CRPs) have been purified to apparent homogeneity by calcium (Ca++) dependent affinity chromatography on phosphoryl choline sepharose column from the sera of L. rohita confined in normal and nonlethal doses of cadmium (Cd ++ ) and mercury (Hg ++ ) polluted water. The CRPs levels are elevated in the serum of both Cd ++ and Hg ++ polluted fishes. All three CRPs are glycoproteins having identical subunits of Mr 33,000. The total content of carbohydrate are 21.4%, 16.4% and 14.2% and that of sialic acid are 3.5%, 2.4% and 1.5% in CRP Hg ++ , CRP Cd ++ and CRP N respectively. In native gel electrophoresis all three CRPs show differences in mobility. However they move to identical position after desialylation and deglycosylation. These CRPs react differentially with different lectins which indicates a variation in the carbohydrate moieties resulting their microheterogeneity.

Document Type: Research Article

Affiliations: Indian Institute of Chemical Biology, 4, Raja S. C. Mallick Road, Calcutta, 700032, India

Publication date: September 1, 1996

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