A Hammerhead Ribozyme That Cleaves Perforin and Fas-ligand RNAs in Vitro
Source: Biochemical and Biophysical Research Communications, Volume 226, Number 3, September 1996 , pp. 595-600(6)
Publisher: Academic Press
Abstract:Polyfunctional ribozymes targeting more than one RNA might be ideal for treatment of diseases caused by the expression of more than one mRNA. This is the case of Graft versus Host Disease, which in the mouse model is due to the action of two proteins responsible for different lytic pathways: perforin and fas-ligand. We have created a bifunctional ribozyme fusing two antiperforin and antifas-ligand hammerhead ribozymes with a stretch of CA dinucleotides. This bifunctional ribozyme is able to recognize and cleave in vitro perforin and fas-ligand mRNA in a specific and selective fashion, with a catalytic efficiency similar to that of its individual components.
Document Type: Research Article
Affiliations: 1: Diabetes Research Institute, University of Miami School of Medicine, Miami, Florida, 33136 2: Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, Florida, 33136
Publication date: September 1, 1996