Protein Expression and Purification

Papers to Appear in Forthcoming Issues
pp. iv-iv(1)

Expression and purification of recombinant full-length NS3 protease–helicase from a new variant of Hepatitis C virus
pp. 363-371(9)
Authors: Poliakov A.; Hubatsch I.; Shuman C.F.; Stenberg G.; Danielson U.H.

A micro-scale process for high-throughput expression of cDNAs in the yeast Saccharomyces cerevisiae
pp. 372-378(7)
Authors: Holz C.; Hesse O.; Bolotina N.; Stahl U.; Lang C.

cDNA cloning, high-level expression, purification, and characterization of an avian Cu,Zn superoxide dismutase from Peking duck
pp. 379-388(10)
Authors: Liu W.; Zhu R-H.; Li G-P.; Wang D-C.

Extraction and purification of decorin from corneal stroma retain structure and biological activity
pp. 389-399(11)
Authors: Brown C.T.; Lin P.; Walsh M.T.; Gantz D.; Nugent M.A.; Trinkaus-Randall V.

High level expression and secretion of truncated forms of herpes simplex virus type 1 and type 2 glycoprotein D by the methylotrophic yeast Pichia pastoris
pp. 400-408(9)
Authors: van Kooij A.; Middel J.; Jakab F.; Elfferich P.; Koedijk D.G.A.M.; Feijlbrief M.; Scheffer A.J.; Degener J.E.; The T.H.; Scheek R.M.; Welling G.W.; Welling-Wester S.

Design, production, and characterization of a monomeric streptavidin and its application for affinity purification of biotinylated proteins
pp. 409-415(7)
Authors: Qureshi M.H.; Wong S-L.

Expression and purification of a small cytokine growth-blocking peptide from armyworm Pseudaletia separata by an optimized fermentation method using the methylotrophic yeast Pichia pastoris
pp. 416-425(10)
Authors: Koganesawa N.; Aizawa T.; Shimojo H.; Miura K.; Ohnishi A.; Demura M.; Hayakawa Y.; Nitta K.; Kawano K.

A purification method of the diagnostic enzyme Bacillus uricase using magnetic beads and non-specific protease
pp. 426-429(4)
Authors: Nishiya Y.; Hibi T.; Oda J-i.

Efficient expression and secretion of recombinant hirudin III in E. coli using the l-asparaginase II signal sequence
pp. 430-436(7)
Authors: Tan S.; Wu W.; Liu J.; Kong Y.; Pu Y.; Yuan R.

Cloning and hemolysin-mediated secretory expression of a codon-optimized synthetic human interleukin-6 gene in Escherichia coli
pp. 437-447(11)
Authors: Li Y.; Chen C.X.; von Specht B-U.; Hahn H.P.

Large-scale production of functional human adrenomedullin: expression, cleavage, amidation, and purification
pp. 448-455(8)
Authors: Mitsuda Y.; Takimoto A.; Kamitani S.; Kitamura K.; Sakata T.; Mitsushima K.

Preparation and expression of biologically active prolactin and growth hormone receptors and suppressor of cytokine signaling proteins 1, 2, 3, and 6 tagged with cyan and yellow fluorescent proteins
pp. 456-464(9)
Authors: Ben-Yair L.; Slaaby R.; Herman A.; Cohen Y.; Biener E.; Moran N.; Yoshimura A.; Whittaker J.; De Meyts P.; Herman B.; Gertler A.

Purification of secreted leukotoxin (LtxA) from Actinobacillus actinomycetemcomitans
pp. 465-471(7)
Authors: Kachlany S.C.; Fine D.H.; Figurski D.H.

Expression and one-step purification of a developmentally regulated protein from Dictyostelium discoideum
pp. 472-480(9)
Authors: Ubeidat M.; Rutherford C.L.

Synthesis of cholera toxin B subunit gene: cloning and expression of a functional 6XHis-tagged protein in Escherichia coli
pp. 481-487(7)
Authors: Paula de Mattos Areˆas A.; Leonor Sarno de Oliveira M.; Raul Romero Ramos C.; Sbrogio-Almeida M.E.; Raw I.; Ho P.L.

Secretory expression and purification of Aspergillus niger glucose oxidase in Saccharomyces cerevisiae mutant deficient in PMR1 gene
pp. 488-493(6)
Authors: Ko J-H.; Hahm M.S.; Kang H.A.; Nam S.W.; Chung B.H.

Large-scale purification of active platelet integrin glycoprotein IIb–IIIa
pp. 494-502(9)
Authors: Hillman M.C.; Zolotarjova N.I.; Patrick D.R.; McCabe D.D.; Shen K.; Corman J.I.; Billheimer J.T.; Seiffert D.A.; Hollis G.F.; Wynn R.

Removal of DnaK contamination during fusion protein purifications
pp. 503-507(5)
Authors: Rial D.V.; Ceccarelli E.A.

Overexpression, purification, and characterization of the periplasmic space thiamin-binding protein of the thiamin traffic ATPase in Escherichia coli
pp. 508-518(11)
Authors: Hollenbach A.D.; Dickson K.A.; Washabaugh M.W.

Expression, purification, and characterization of recombinant nonphosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Clostridium acetobutylicum
pp. 519-526(8)
Authors: Iddar A.; Valverde F.; Serrano A.; Soukri A.

Expression, purification, and characterization of human dipeptidyl peptidase IV/CD26 in Sf9 insect cells
pp. 527-532(6)
Authors: Dobers J.; Zimmermann-Kordmann M.; Leddermann M.; Schewe T.; Reutter W.; Fan H.

Expression, purification, and biochemical characterization of Mycobacterium tuberculosis aspartate decarboxylase, PanD
pp. 533-540(8)
Authors: Chopra S.; Pai H.; Ranganathan A.

Preparation of active recombinant cathepsin K expressed in bacteria as inclusion body
pp. 541-546(6)
Authors: Hwang H-S.; Chung H-S.

Heterologous expression, purification, and enzymatic activity of Mycobacterium tuberculosis NAD⁺ synthetase
pp. 547-557(11)
Authors: Bellinzoni M.; De Rossi E.; Branzoni M.; Milano A.; Peverali F.A.; Rizzi M.; Riccardi G.

Author Index for Volume 25
pp. 558-559(2)