Purification of NAD glycohydrolase from Agkistrodon acutus venom
Authors: Wu S.D.1; Liu Y.1; Xu X.2; Zhu Z.1
Source: Protein Expression and Purification, Volume 25, Number 2, July 2002 , pp. 319-322(4)
Publisher: Academic Press
- In this: publication
- By this: publisher
- In this Subject: Anatomy & Physiology
- By this author: Wu S.D. ; Liu Y. ; Xu X. ; Zhu Z.
Abstract:
NAD glycohydrolase (NADase) from Agkistrodon acutus venom was purified to electrophoretic homogeneity by a fast, reproducible 3-step procedure including Q Sepharose Fast Flow, Superdex 75, and Mono S column chromatography. This new procedure gave a 15.6-fold purification with a recovery yield of 7.9% and a specific activity of 12.8 units/mg.
© 2002 Elsevier Science (USA)
Language: English
Document Type: Research article
Affiliations: 1: Laboratory of Internal Friction and Defects in Solids, Institute of Solid State Physics, Chinese Academy of Sciences, Hefei 230031, China 2: Department of Chemistry, University of Science and Technology of China, Hefei 230036, China

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