Purification of NAD glycohydrolase from Agkistrodon acutus venom

Authors: Wu S.D.¹; Liu Y.¹; Xu X.²; Zhu Z.¹

Source: Protein Expression and Purification, Volume 25, Number 2, July 2002 , pp. 319-322(4)

Publisher: Academic Press

Abstract:

NAD glycohydrolase (NADase) from Agkistrodon acutus venom was purified to electrophoretic homogeneity by a fast, reproducible 3-step procedure including Q Sepharose Fast Flow, Superdex 75, and Mono S column chromatography. This new procedure gave a 15.6-fold purification with a recovery yield of 7.9% and a specific activity of 12.8 units/mg.

© 2002 Elsevier Science (USA)

Language: English

Document Type: Research article

Affiliations: 1: Laboratory of Internal Friction and Defects in Solids, Institute of Solid State Physics, Chinese Academy of Sciences, Hefei 230031, China 2: Department of Chemistry, University of Science and Technology of China, Hefei 230036, China

Publication date: 2002-07-01

• In this: publication
• By this: publisher
• In this Subject: Anatomy & Physiology
• By this author: Wu S.D. ;  Liu Y. ;  Xu X. ;  Zhu Z.

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