Protein Expression and Purification

High Throughput Methods for Gene Cloning and Expression
pp. 1-7(7)
Authors: Dieckman L.; Gu M.; Stols L.; Donnelly M.I.; Collart F.R.

A New Vector for High-Throughput, Ligation-Independent Cloning Encoding a Tobacco Etch Virus Protease Cleavage Site
pp. 8-15(8)
Authors: Stols L.; Gu M.; Dieckman L.; Raffen R.; Collart F.R.; Donnelly M.I.

Expression and Purification of the Recombinant Enteropathogenic Escherichia coli Vaccine Candidates BfpA and EspB
pp. 16-22(7)
Authors: Quintana Flores V.M.; Campos de Souza Fernandes R.C.; Sousa de Macedo Z.; Medina-Acosta E.

In Vitro Assembly, Purification, and Crystallization of the Rab Geranylgeranyl Transferase:Substrate Complex
pp. 23-30(8)
Authors: Rak A.; Niculae A.; Kalinin A.; Thomä N.H.; Sidorovitch V.; Goody R.S.; Alexandrov K.

Optimization of Baculovirus-Mediated Expression and Purification of Hexahistidine-Tagged Murine DNA (Cytosine-C5)-Methyltransferase-1 in Spodoptera frugiperda 9 Cells
pp. 31-40(10)
Authors: Brank A.S.; Van Bemmel D.M.; Christman J.K.

Expression, Characterization, and Purification of Recombinant Porcine Lactoferrin in Pichia pastoris
pp. 41-49(9)
Authors: Wang S-H.; Yang T-S.; Lin S-M.; Tsai M-S.; Wu S-C.; Mao S.J.T.

Purification of -Sarcin and an Antifungal Protein from Mold (Aspergillus giganteus) by Chitin Affinity Chromatography
pp. 50-58(9)
Authors: Liu R-s.; Huang H.; Yang Q.; Liu W-Y.

Crocodilian -Crystallin: Overexpression, Purification, and Characterization
pp. 59-64(6)
Authors: Mishra A.K.; Chandrashekhar R.; Aggarwal R.K.; Sharma Y.

Purification and Characterization of Recombinant Plasmodium falciparum Adenylosuccinate Synthetase Expressed in Escherichia coli
pp. 65-72(8)
Authors: Jayalakshmi R.; Sumathy K.; Balaram H.

Expression of Recombinant Human Betaine: Homocysteine S-Methyltransferase for X-Ray Crystallographic Studies and Further Characterization of Interaction with S-Adenosylmethionine
pp. 73-80(8)
Authors: Bose N.; Greenspan P.; Momany C.

Expression and Purification of Two Hydrophobic Double-Spanning Membrane Proteins Derived from the Cystic Fibrosis Transmembrane Conductance Regulator
pp. 81-86(6)
Authors: Therien A.G.; Glibowicka M.; Deber C.M.

Large-Scale Purification and Characterization of Malaria Vaccine Candidate Antigen Pvs25H for Use in Clinical Trials
pp. 87-96(10)
Authors: Miles A.P.; Zhang Y.; Saul A.; Stowers A.W.

Large-Scale Expression and Purification of High-Molecular-Weight Glutenin Subunits
pp. 97-104(8)
Authors: Dowd C.; Bekes F.

Expression, Refolding, Purification, Molecular Characterization, Crystallization, and Preliminary X-ray Analysis of the Receptor Binding Domain of Human B7-2
pp. 105-113(9)
Authors: Zhang X.; Schwartz J-C.D.; Almo S.C.; Nathenson S.G.

Rescue of Intracellularly Trapped Lutropin Receptor Exodomain by Endodomain and Reconstitution of a Functional Membrane Receptor: Interaction between Exo- and Endodomains
pp. 114-123(10)
Authors: Bozon V.; Couture L.; Pajot-Augy E.; Richard F.; Remy J-J.; Salesse R.

Bacterial Expression of a Natively Folded Extracellular Domain Fusion Protein of the hFSH Receptor in the Cytoplasm of Escherichia coli
pp. 124-133(10)
Authors: Lobel L.; Pollak S.; Lustbader B.; Klein J.; Lustbader J.W.

A Rapid Selective Extraction Procedure for the Outer Membrane Protein (OmpF) from Escherichia coli
pp. 134-137(4)
Authors: Arcidiacono S.; Butler M.M.; Mello C.M.

Refolding and Purification of Recombinant Human PDE7A Expressed in Escherichia coli as Inclusion Bodies
pp. 138-148(11)
Authors: Richter W.; Hermsdorf T.; Kronbach T.; Dettmer D.

Purification and Biochemical Characterization of the ErmSF Macrolide-Lincosamide-Streptogramin B Resistance Factor Protein Expressed as a Hexahistidine-Tagged Protein in Escherichia coli
pp. 149-159(11)
Authors: Jin H.J.; Yang Y.D.

Expression and Production of Recombinant Human Interleukin-2 in Potato Plants
pp. 160-165(6)
Authors: Park Y.; Cheong H.

Bacterial Expression and in Vitro Refolding of a Single-Chain Fv Antibody Specific for Human Plasma Apolipoprotein B-100
pp. 166-173(8)
Authors: Lee M-H.; Park T-I.; Park Y-B.; Kwak J-W.

Chromatographic Methods for the Isolation of, and Refolding of Proteins from, Escherichia coli Inclusion Bodies
pp. 174-179(6)
Authors: Gu Z.; Weidenhaupt M.; Ivanova N.; Pavlov M.; Xu B.; Su Z-G.; Janson J-C.

The Interaction of Recombinant Decorin with ₂HS-Glycoprotein—Implications for Structural and Functional Investigations
pp. 180-188(9)
Authors: Sugars R.V.; Waddington R.J.; Embery G.

Expression and Purification of Aspartate -Semialdehyde Dehydrogenase from Infectious Microorganisms
pp. 189-194(6)
Authors: Moore R.A.; Bocik W.E.; Viola R.E.

Expression and Affinity Purification of Recombinant Proteins from Plants
pp. 195-202(8)
Authors: Desai U.A.; Sur G.; Daunert S.; Babbitt R.; Li Q.

Cloning, Overexpression, Purification, and Matrix-Assisted Refolding of DevS (Rv 3132c) Histidine Protein Kinase of Mycobacterium tuberculosis
pp. 203-208(6)
Authors: Saini D.K.; Pant N.; Das T.K.; Tyagi J.S.