Protein Expression and Purification

Affinity-Purification of Transferrin-Binding Protein B under Nondenaturing Conditions
pp. 323-328(6)
Authors: Krell T.; Chevalier M.; Lissolo L.

Roles of NADPH-P450 Reductase and Apo- and Holo-Cytochrome b₅ on Xenobiotic Oxidations Catalyzed by 12 Recombinant Human Cytochrome P450s Expressed in Membranes of Escherichia coli
pp. 329-337(9)
Authors: Yamazaki H.; Nakamura M.; Komatsu T.; Ohyama K.; Hatanaka N.; Asahi S.; Shimada N.; Guengerich F.P.; Shimada T.; Nakajima M.; Yokoi T.

Soluble Expression of a Functional Recombinant Cytolytic Immunotoxin in Insect Cells
pp. 338-347(10)
Authors: Choo A.B.H.; Dunn R.D.; Broady K.W.; Raison R.L.

Secretory Production and Purification of Functional Full-Length Streptavidin from Bacillus subtilis
pp. 348-356(9)
Authors: Wu S-C.; Hassan Qureshi M.; Wong S-L.

Design, Production, and Characterization of an Engineered Biotin Ligase (BirA) and Its Application for Affinity Purification of Staphylokinase Produced from Bacillus subtilis via Secretion
pp. 357-365(9)
Authors: Wu S-C.; Yeung J.C.; Hwang P.M.; Wong S-L.

Expression in Escherichia coli and Purification of Hexahistidine-Tagged Human Tissue Transglutaminase
pp. 366-373(8)
Authors: Shi Q.; Kim S-Y.; Blass J.P.; Cooper A.J.L.

Expression, Purification, and Isotope Labeling of a gp120 V3 Peptide and Production of a Fab from a HIV-1 Neutralizing Antibody for NMR Studies
pp. 374-383(10)
Authors: Sharon M.; Görlach M.; Levy R.; Hayek Y.; Anglister J.

Combined Use of Regulatory Elements within the cDNA to Increase the Production of a Soluble Mouse Single-Chain Antibody, scFv, from Tobacco Cell Suspension Cultures
pp. 384-394(11)
Authors: Xu H.; Montoya F.U.; Wang Z.; Lee J.M.; Reeves R.; Linthicum D.S.; Magnuson N.S.

Expression and Refolding of Recombinant Human -Tocopherol Transfer Protein Capable of Specific -Tocopherol Binding
pp. 395-403(9)
Authors: Panagabko C.; Morley S.; Neely S.; Lei H.; Manor D.; Atkinson J.

Osmoregulatory Isoform of Dihydroxyacetone Phosphate Reductase from Dunaliella tertiolecta: Purification and Characterization
pp. 404-411(8)
Authors: Ghoshal D.; Mach D.; Agarwal M.; Goyal A.; Goyal A.

Utility of (His)₆ Tag for Purification and Refolding of Proplasmepsin-2 and Mutants with Altered Activation Properties
pp. 412-419(8)
Authors: Gulnik S.V.; Afonina E.I.; Gustchina E.; Yu B.; Silva A.M.; Kim Y.; Erickson J.W.

Expression, Purification, and Structural Characterization of Human Histone H4
pp. 420-428(9)
Authors: Vergani L.; Canneva F.; Ghisellini P.; Nicolini C.

A Flavoprotein Encoded in Selenomonas ruminantium Is Characterized after Expression in Escherichia coli
pp. 429-438(10)
Authors: Anderson P.J.; Cole L.J.; McKay D.B.; Entsch B.

Tabtoxin-Resistant Protein: Overexpression, Purification, and Characterization
pp. 439-444(6)
Authors: Liu J.; Le Y.; Ye B.; Zhen Y.; Zhu C.; Shen J.; Zhang R.

Overexpression, Purification, and Biochemical Characterization of the Extracellular Human CD83 Domain and Generation of Monoclonal Antibodies
pp. 445-452(8)
Authors: Lechmann M.; Kremmer E.; Sticht H.; Steinkasserer A.

High-Level Expression and Secretion of Recombinant Mouse Endostatin by Escherichia coli
pp. 453-459(7)
Authors: Xu R.; Du P.; Fan J-J.; Zhang Q.; Li T-P.; Gan R-B.

Affinity Extraction of Dye- and Metal Ion-Binding Proteins in Polyvinylpyrrolidone-Based Aqueous Two-Phase System
pp. 460-469(10)
Authors: Fernandes S.; Kim H-S.; Hatti-Kaul R.

Effect of Tandem Rare Codon Substitution and Vector-Host Combinations on the Expression of the EBV gp110 C-Terminal Domain in Escherichia coli
pp. 470-480(11)
Authors: Park S.J.; Lee S.K.; Lee B.J.

A Mammalian Expression System for Rapid Production and Purification of Active MAP Kinase Phosphatases
pp. 481-488(8)
Authors: Chen P.; Hutter D.; Liu P.; Liu Y.

Purification and Characterization of the Major Lipoprotein (P28) of Spiroplasma apis
pp. 489-496(8)
Authors: Le Hénaff M.; Crémet J-Y.; Fontenelle C.

Expression and Purification of Homogenous Proteins in Saccharomyces cerevisiae Based on Ubiquitin-FLAG Fusion
pp. 497-504(8)
Authors: Einhauer A.; Schuster M.; Wasserbauer E.; Jungbauer A.

Characterization of an Antibody Fv Fragment That Binds to the Human, but Not to the Rat Neurotensin Receptor NTS-1
pp. 505-512(8)
Authors: Grisshammer R.; Grunwald T.; Sohal A.K.

Expression of FLAG Fusion Proteins in Insect Cells: Application to the Multi-subunit Transcription/DNA Repair Factor TFIIH
pp. 513-523(11)
Authors: Jawhari A.; Uhring M.; Crucifix C.; Fribourg S.; Schultz P.; Poterszman A.; Egly J.M.; Moras D.

Delandin, a Chitinase-like Protein with Antifungal, HIV-1 Reverse Transcriptase Inhibitory and Mitogenic Activities from the Rice Bean Delandia umbellata
pp. 524-529(6)
Authors: Ye X.Y.; Ng T.B.

An X-Prolyl Dipeptidyl Aminopeptidase from Lactococcus lactis: Cloning, Expression in Escherichia coli, and Application for Removal of N-Terminal Pro-Pro from Recombinant Proteins
pp. 530-538(9)
Authors: Xin M.; Li Y.; Jie L.; Min D.; Liu J.

Author Index for Volume 24
pp. 540-541(2)