Protein Expression and Purification

Immunoaffinity Purification and Reconstitution of Human ₂-Adrenergic Receptor Subtype C2 into Phospholipid Vesicles
pp. 1-10(10)
Authors: Liitti S.; Matikainen M-T.; Scheinin M.; Glumoff T.; Goldman A.

Expression and Purification of Monospecific and Bispecific Recombinant Antibody Fragments Derived from Antibodies That Block the CD80/CD86-CD28 Costimulatory Pathway
pp. 11-24(14)
Authors: Dincq S.; Bosman F.; Buyse M-A.; Degrieck R.; Celis L.; Boer M.d.; Van Doorsselaere V.; Sablon E.

Expression of Giardia duodenalis -Tubulin as a Soluble Protein in Escherichia coli
pp. 25-30(6)
Authors: MacDonald L.M.; Armson A.; Thompson R.C.A.; Reynoldson J.A.

Two-Cistron System Overexpression of Chloroplast Glyceraldehyde-3-phosphate Dehydrogenase Subunit B and B-Derivatives from Spinach in Escherichia coli
pp. 31-37(7)
Authors: Tang G-L.; Wang Y-F.; Bao J-S.; Chen H-B.

One-Step Purification of a Fully Active Hexahistidine-Tagged Human Hexokinase Type I Overexpressed in Escherichia coli
pp. 38-44(7)
Authors: Palma F.; Longhi S.; Agostini D.; Stocchi V.

Systematic Separation and Purification of Elastase, Gelatinase (Matrix Metalloproteinase 9), and Collagenase (Matrix Metalloproteinase 8) from Polymorphonuclear Leukocytes in Dialyzers Previously Used by Patients with Renal Failure
pp. 45-51(7)
Authors: Kobayashi T.; Nishikawa T.; Hattori S.; Yoshida N.; Takagi T.; Watanabe H.; Hori H.; Nagai Y.

Subcloning, Expression, Purification, and Characterization of Haemophilus influenzae Glycerol Kinase
pp. 52-59(8)
Authors: Pawlyk A.C.; Pettigrew D.W.

Recombinant Expression of Biologically Active Rat Leptin in Escherichia coli
pp. 60-69(10)
Authors: Park J-H.; Lee H-H.; Na S-Y.; Ju S-K.; Lee Y-J.; Lee M-K.; Kim K.L.

The Expression of a Single-Chain Fv Antibody Fragment in Different Plant Hosts and Tissues by Using Potato Virus X as a Vector
pp. 70-74(5)
Authors: Roggero P.; Ciuffo M.; Benvenuto E.; Franconi R.

Expression and Purification of Escherichia coli -Glucuronidase
pp. 75-81(7)
Authors: Aich S.; Delbaere L.T.J.; Chen R.

Inexpensive Purification of P450 Reductase and Other Proteins Using 2,5-Adenosine Diphosphate Agarose Affinity Columns
pp. 82-83(2)
Authors: Rock D.; Rock D.; Jones J.P.

Expression of Mammalian Geranylgeranyltransferase Type-II in Escherichia coli and Its Application for in Vitro Prenylation of Rab Proteins
pp. 84-91(8)
Authors: Kalinin A.; Thomä N.H.; Iakovenko A.; Heinemann I.; Rostkova E.; Constantinescu A.T.; Alexandrov K.

Recombinant Human Tumor Antigen MUC1 Expressed in Insect Cells: Structure and Immunogenicity
pp. 92-100(9)
Authors: Soares M.; Hanisch F-G.; Finn O.J.; Ciborowski P.

Refolding and Purification of Yeast Carboxypeptidase Y Expressed as Inclusion Bodies in Escherichia coli
pp. 101-107(7)
Authors: Hahm M.S.; Chung B.H.

Accurate Disulfide Formation in Escherichia coli: Overexpression and Characterization of the First Domain (HF6478) of the Multiple Kazal-Type Inhibitor LEKTI
pp. 108-112(5)
Authors: Lauber T.; Marx U.C.; Schulz A.; Kreutzmann P.; Rösch P.; Hoffmann S.

Construction and Expression of an Enzymatically Active Form of PECAM-1 Containing the Phosphatase Domain of the Protein Tyrosine Phosphatase, SHP-2
pp. 113-119(7)
Authors: Noda M.; Paddock C.; Newman P.J.

Overexpression, Rapid Isolation, and Biochemical Characterization of Escherichia coli Single-Stranded DNA-Binding Protein
pp. 120-127(8)
Authors: Ehn M.; Nilsson P.; Uhlén M.; Hober S.

Systematic Optimization of Expression and Refolding of the Plasmodium falciparum Cysteine Protease Falcipain-2
pp. 128-134(7)
Authors: Sijwali P.S.; Brinen L.S.; Rosenthal P.J.

Intein-Mediated Rapid Purification of Cre Recombinase
pp. 135-140(6)
Authors: Cantor E.J.; Chong S.

Expression in Spodoptera frugiperda (Sf21) Insect Cells of BT-R₁, a Cadherin-Related Receptor from Manduca sexta for Bacillus thuringiensis Cry1Ab Toxin
pp. 141-147(7)
Authors: Meng J.; Candas M.; Keeton T.P.; Bulla L.A.

Isolation, Expression, and Characterization of Fully Functional Nontoxic BiP/GRP78 Mutants
pp. 148-158(11)
Authors: King L.; Berg M.; Chevalier M.; Carey A.; Elguindi E.C.; Blond S.Y.