Purification of a Fragment of MDM2 for Production of Polyclonal Antisera

Authors: Carstens B.P.; Perry M.E.

Source: Protein Expression and Purification, Volume 21, Number 2, March 2001 , pp. 361-365(5)

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Abstract:

A histidine-tagged, carboxy-terminal fragment of the murine double minute 2 gene product, p90^MDM2, was purified by Ni-NTA chromatography and preparative gel electrophoresis. The purified MDM2 fragment was used to generate polyclonal antisera that recognize multiple species of MDM2 proteins, including the inhibitor of p53, p90^MDM2, as well as the activator of p53, p76^MDM2. The antibodies are useful for Western analysis, immunoprecipitation, and immunofluorescence. Copyright 2001 Academic Press.

Language: English

Document Type: Research article

Affiliations: Department of Oncology, University of Wisconsin Medical School, Madison, Wisconsin, 53706

Publication date: 2001-03-01